Feasibility of NAD(P)/NAD(P)H as redox agents in enzymatic plasmonic gold nanostar assays for galactose quantification.
| Autor: | Munyayi TA; Centre For Human Metabolomics, Department of Biochemistry, North West University Potchefstroom, 11 Hoffman Street, Potchefstroom 2531, South Africa., Mulder DW; Centre For Human Metabolomics, Department of Biochemistry, North West University Potchefstroom, 11 Hoffman Street, Potchefstroom 2531, South Africa., Conradie EH; Centre For Human Metabolomics, Department of Biochemistry, North West University Potchefstroom, 11 Hoffman Street, Potchefstroom 2531, South Africa., Vorster BC; Centre For Human Metabolomics, Department of Biochemistry, North West University Potchefstroom, 11 Hoffman Street, Potchefstroom 2531, South Africa. |
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| Jazyk: | angličtina |
| Zdroj: | Royal Society open science [R Soc Open Sci] 2023 Oct 11; Vol. 10 (10), pp. 230825. Date of Electronic Publication: 2023 Oct 11 (Print Publication: 2023). |
| DOI: | 10.1098/rsos.230825 |
| Abstrakt: | Plasmonic colorimetric sensors have emerged as powerful analytical tools in biochemistry due to their localized surface plasmon resonance extinction in the visible range. Here, we describe the feasibility of NAD(P)/NAD(P)H as redox agents in enzymatic plasmonic gold nanostar (AuNS) assays for galactose quantification using three model enzymes, GalDH, AR and GalOx, immobilized separately on polyvinylpyrrolidone-capped AuNS scaffolds. These highly specific, sensitive and selective bioassays induce the transformation of AuNS into quasi-spherical nanoparticles during the biorecognition of galactose in water and synthetic blood matrices. As a result, using our inexpensive and simple AuNS plasmon bioassays, the presence of galactose may be detected spectrophotometrically and by the naked eye. Competing Interests: We have no competing interests to declare. (© 2023 The Authors.) |
| Databáze: | MEDLINE |
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