"Mini-community" simulation revealed the differences of endophytic fungal communities between the above- and below-ground tissues of Ephedra sinica Stapf.

Autor: Liu X; Institute of Applied Chemistry, Shanxi University, Taiyuan, Shanxi, 030006, People's Republic of China; Modern Research Center for Traditional Chinese Medicine, The Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan, Shanxi, 030006, People's Republic of China., Cui JL; Institute of Applied Chemistry, Shanxi University, Taiyuan, Shanxi, 030006, People's Republic of China; State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs, Beijng, 100700, People's Republic of China. Electronic address: CJL717@163.com., Zhou ZY; Institute of Applied Chemistry, Shanxi University, Taiyuan, Shanxi, 030006, People's Republic of China; Modern Research Center for Traditional Chinese Medicine, The Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan, Shanxi, 030006, People's Republic of China., Zhang ZB; School of Chemistry and Chemical Engineering, Shanxi University, Taiyuan, Shanxi, 030006, People's Republic of China., Cui XG; School of Chemistry and Chemical Engineering, Shanxi University, Taiyuan, Shanxi, 030006, People's Republic of China., Han B; Institute of Applied Chemistry, Shanxi University, Taiyuan, Shanxi, 030006, People's Republic of China; Modern Research Center for Traditional Chinese Medicine, The Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan, Shanxi, 030006, People's Republic of China., Wang JH; Institute of Applied Chemistry, Shanxi University, Taiyuan, Shanxi, 030006, People's Republic of China., Wang ML; Institute of Applied Chemistry, Shanxi University, Taiyuan, Shanxi, 030006, People's Republic of China., Li YM; College of Pharmacy, Shaanxi University of Chinese Medicine, Xianyang, Shaanxi, 712046, People's Republic of China. Electronic address: 2051058@sntcm.edu.cn.
Jazyk: angličtina
Zdroj: Fungal biology [Fungal Biol] 2023 Sep; Vol. 127 (9), pp. 1276-1283. Date of Electronic Publication: 2023 Jul 26.
DOI: 10.1016/j.funbio.2023.07.006
Abstrakt: The microecology of endophytic fungi in special habitats, such as the interior of different tissues from a medicinal plant, and its effects on the formation of metabolites with different biological activities are of great importance. However, the factors affecting fungal community formation are unclear. This study is the first to utilize "mini-community" remodeling to understand the above phenomena. First, high-throughput sequencing technology was applied to explore the community composition and diversity of endophytic fungi in the above-ground tissues (Ea) and below-ground tissues (Eb) of Ephedra sinica. Second, fungi were obtained through culture-dependent technology and used for "mini-community" remodeling in vitro. Then, the effects of environmental factors, partner fungi, and plant tissue fluid (internal environment) on endophytic fungal community formation were discussed. Results showed that environmental factors played a decisive role in the selection of endophytic fungi, that is, in Ea and Eb, 93.8% and 25.3% of endophytic fungi were halophilic, respectively, and 10.6% and 60.2% fungi were sensitive to high temperature (33 °C), respectively. Meanwhile, pH had little effect on fungal communities. The internal environment of the plant host further promoted the formation of endophytic fungal communities.
Competing Interests: Declaration of competing interest The authors declare that they have no competing interests.
(Copyright © 2023 British Mycological Society. Published by Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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