Prediction of the bacterial shape in urinary tract infections with the Sysmex UF-1000i analyser: technical note.

Autor: Benhamza N; Laboratory of Microbiology, Mohamed VI Teaching Hospital., Aarab A; Laboratory of Microbiology, Mohamed VI Teaching Hospital., Farih S; Laboratory of Microbiology, Mohamed VI Teaching Hospital., Saddari A; Laboratory of Microbiology, Mohamed VI Teaching Hospital., Yacoubi L; Laboratory of Microbiology, Mohamed VI Teaching Hospital., Benaissa E; Department of Bacteriology, Mohammed V Teaching Military Hospital.; Epidemiology and Bacterial Resistance Research Team/BIO-INOVA Centre, Faculty of Medicine and Pharmacy, University Mohammed V, Rabat, Morocco., Ben Lahlou Y; Department of Bacteriology, Mohammed V Teaching Military Hospital.; Epidemiology and Bacterial Resistance Research Team/BIO-INOVA Centre, Faculty of Medicine and Pharmacy, University Mohammed V, Rabat, Morocco., Elouennass M; Epidemiology and Bacterial Resistance Research Team/BIO-INOVA Centre, Faculty of Medicine and Pharmacy, University Mohammed V, Rabat, Morocco.; Department of Bacteriology, Mohammed V Teaching Military Hospital., Maleb A; Laboratory of Microbiology, Mohamed VI Teaching Hospital.; Research team 'Cell Biology and Pharmacology Applied to Health Sciences', Faculty of Medicine and Pharmacy, Mohammed First University, Oujda.
Jazyk: angličtina
Zdroj: Annals of medicine and surgery (2012) [Ann Med Surg (Lond)] 2023 Jul 27; Vol. 85 (10), pp. 4877-4881. Date of Electronic Publication: 2023 Jul 27 (Print Publication: 2023).
DOI: 10.1097/MS9.0000000000000701
Abstrakt: Background: The aim of our study was to explore the utility of the Sysmex UF-1000i analyzer as a rapid screening tool for urinary tract infections (UTI) and its ability to predict bacterial shape in order to help physicians choose the appropriate empiric treatment.
Materials and Methods: This is a retrospective study, including 1023 urine cytobacteriological examinations. Urines were processed according to the recommendations of the medical microbiology reference system (REMIC). Using the Sysmex Uf-1000i analyzer, the authors evaluated bacteria forward scatter (B_FSC) and fluorescent light scatter (B_FLH) in a preliminary discrimination step for UTI caused by bacilli or cocci bacteria.
Results: The authors got 1023 positive samples. Comparing baccili and cocci bacteria, the authors observed a statistically significant difference for B_FSC but not for B_FLH. The values of B_FLH are very close for the four categories of microorganisms compared (bacilli, cocci, bacilli-cocci association, and yeasts). For these same categories, tests show different values for the B_FSC. A separate analysis of the B_FSC values for bacilli shows that their distribution is relatively homogeneous and exhibits a peak between 20 and 30 ch.
Conclusion: Dimensional parameters of bacteria generated by UF-1000i could be a rapid and useful tool for predicting the bacterial shape causing UTI.
Competing Interests: The authors declare that they have no conflicts of interest.Sponsorships or competing interests that may be relevant to content are disclosed at the end of this article.
(Copyright © 2023 The Author(s). Published by Wolters Kluwer Health, Inc.)
Databáze: MEDLINE