A comprehensive painkillers screening by assessing zebrafish behaviors after caudal fin amputation.

Autor: Audira G; Department of Bioscience Technology, Chung Yuan Christian University, Taoyuan 320314, Taiwan., Huang JC; Department of Applied Chemistry, National Pingtung University, Pingtung 90003, Taiwan., Chen KH; Department of Applied Chemistry, National Pingtung University, Pingtung 90003, Taiwan., Kurnia KA; Department of Bioscience Technology, Chung Yuan Christian University, Taoyuan 320314, Taiwan; Department of Applied Chemistry, National Pingtung University, Pingtung 90003, Taiwan; Department of Chemistry, Chung Yuan Christian University, Taoyuan 320314, Taiwan., Vasquez RD; Department of Pharmacy, Research Center for Natural and Applied Sciences, University of Santo Tomas, Manila 1008, Philippines., Roldan MJM; Faculty of Pharmacy, The Graduate School, University of Santo Tomas, Manila 1008, Philippines., Lai YH; Department of Chemistry, Chinese Culture University, Taipei 11114, Taiwan., Hsiao CD; Department of Bioscience Technology, Chung Yuan Christian University, Taoyuan 320314, Taiwan; Department of Chemistry, Chung Yuan Christian University, Taoyuan 320314, Taiwan; Center for Nanotechnology, Chung Yuan Christian University, Taoyuan 320314, Taiwan; Research Center for Aquatic Toxicology and Pharmacology, Chung Yuan Christian University, Taoyuan 320314, Taiwan. Electronic address: cdhsiao@cycu.edu.tw., Yen CY; Department of Orthopedics, E-Da Cancer Hospital, Kaohsiung, Taiwan; School of Medicine, College of Medicine, I-Shou University, No.1, E-Da Road, Yan-Chau District, 824, Kaohsiung, Taiwan. Electronic address: isu19550@isu.edu.tw.
Jazyk: angličtina
Zdroj: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie [Biomed Pharmacother] 2023 Dec; Vol. 168, pp. 115641. Date of Electronic Publication: 2023 Oct 06.
DOI: 10.1016/j.biopha.2023.115641
Abstrakt: Recently, the usage of zebrafish for pain studies has increased in the past years, especially due to its robust pain-stimulated behaviors. Fin amputation has been demonstrated to induce a noxious response in zebrafish. However, based on the prior study, although lidocaine, the most used painkiller in zebrafish, has been shown to ameliorate amputated zebrafish behaviors, it still causes some prolonged effects. Therefore, alternative painkillers are always needed to improve the treatment quality of fin-amputated zebrafish. Here, the effects of several analgesics in recovering zebrafish behaviors post-fin amputation were evaluated. From the results, five painkillers were found to have potentially beneficial effects on amputated fish behaviors. Overall, these results aligned with their binding energy level to target proteins of COX-1 and COX-2. Later, based on their sub-chronic effects on zebrafish survivability, indomethacin, and diclofenac were further studied. This combination showed a prominent effect in recovering zebrafish behaviors when administered orally or through waterborne exposure, even with lower concentrations. Next, based on the ELISA in zebrafish brain tissue, although some changes were found in the treated group, no statistical differences were observed in most of the tested biomarkers. However, since heatmap clustering showed a similar pattern between biochemical and behavior endpoints, the minor changes in each biomarker may be sufficient in changing the fish behaviors.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 The Authors. Published by Elsevier Masson SAS.. All rights reserved.)
Databáze: MEDLINE
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