| Autor: |
Mehmood A; Department of Botany, University of Poonch Rawalakot, Rawalakot, Azad Kashmir, Pakistan., Zahir S; Department of Botany, University of Poonch Rawalakot, Rawalakot, Azad Kashmir, Pakistan., Rauf Khan MA; Department of Physics, University of Poonch Rawalakot, Rawalakot, Azad Kashmir, Pakistan., Ahmad KS; Department of Botany, University of Poonch Rawalakot, Rawalakot, Azad Kashmir, Pakistan., Abasi F; Department of Botany, PMAS-Arid Agriculture University, Rawalpindi, Punjab, Pakistan., Raffi M; Pakistan Institute of Engineering and Applied Sciences, National Institute of Lasers and Optronics College, Nilore, Islamabad, Pakistan., Proćków J; Department of Plant Biology, Institute of Environmental Biology, Wrocław University of Environmental and Life Sciences, Wrocław, Poland., M Pérez de la Lastra J; Biotechnology of Macromolecules Research Group, Department of Life and Earth Sciences, Instituto de Productos Naturales y Agrobiología-Consejo Superior de Investigaciones Científicas, (IPNA-CSIC), Tenerife, Spain. |
| Abstrakt: |
This report examines the bio-fabrication of silver nanoparticles (Ag-NPs) utilizing AgNO 3 and leaf extract of Crataegus monogyna as the precursor material. In order to maximize the antibacterial efficacy against Staphylococcus aureus , Proteus mirabilis , Klebsiella pneumoniae and Pseudomonas aeruginosa , the reaction conditions for the green fabrication of Ag-NPs were optimized. A one factor at a time approach (volume concentration of extract, volume concentration of AgNO 3 , pH and temperature) was used to optimize the best condition, and results were assessed through UV-visible spectroscopy and particle size distribution. The results showed that 20 mL of plant extract, 80 mL of AgNO 3 , pH 08, 100 °C temperature were the optimum reaction conditions under which we obtained the smallest Ag-NPs (7 nm). The scanning electron microscopy and X-ray diffraction analysis confirmed the spherical and crystalline nature of Ag-NPs. The antibacterial activity assay demonstrated a high antibacterial effect of Ag-NPs against S. aureus , P. mirabilis , K. pneumoniae and P. aeruginosa , and that impact was greater with smaller-sized nanoparticles (7 nm). This study shows that leaf extract of C. monogyna is a possible medium for the green fabrication of Ag-NPs, and control over reaction factors can establish the characteristics and antibacterial effectiveness of Ag-NPs.Communicated by Ramaswamy H. Sarma. |
