Shared bias in H chain V-J pairing in naive and memory B cells.

Autor: Levi R; Department of Mathematics, Bar Ilan University, Ramat Gan, Israel., Dvorkin S; Department of Mathematics, Bar Ilan University, Ramat Gan, Israel., Louzoun Y; Department of Mathematics, Bar Ilan University, Ramat Gan, Israel.
Jazyk: angličtina
Zdroj: Frontiers in immunology [Front Immunol] 2023 Sep 18; Vol. 14, pp. 1166116. Date of Electronic Publication: 2023 Sep 18 (Print Publication: 2023).
DOI: 10.3389/fimmu.2023.1166116
Abstrakt: Introduction: H chain rearrangement in B cells is a two-step process where first D H binds J H , and only then V H is joined to the complex. As such, there is no direct rearrangement between V H and J H .
Results: Nevertheless, we here show that the V H JH combinations frequency in humans deviates from the one expected based on each gene usage frequency. This bias is observed mainly in functional rearrangements, and much less in out-of-frame rearrangements. The bias cannot be explained by preferred binding for D H genes or a preferred reading frame. Preferred V H J H combinations are shared between donors.
Discussion: These results suggest a common structural mechanism for these biases. Through development, thepreferred V H J H combinations evolve during peripheral selection to become stronger, but less shared. We propose that peripheral Heavy chain V H J H usage is initially shaped by a structural selection before the naive B cellstate, followed by pathogen-induced selection for host specific V H - J H pairs.
Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
(Copyright © 2023 Levi, Dvorkin and Louzoun.)
Databáze: MEDLINE
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