Avian sarcoma/leukosis virus (RCAS)-mediated over-expression of IFITM3 protects chicks from highly pathogenic avian influenza virus subtype H5N1.

Autor: Rohaim MA; Division of Biomedical and Life Sciences, Faculty of Health and Medicine, Lancaster University, Lancaster LA1 4YG, UK; Department of Virology, Faculty of Veterinary Medicine, Cairo University, Giza 12211, Egypt., Gardiner EL; Division of Biomedical and Life Sciences, Faculty of Health and Medicine, Lancaster University, Lancaster LA1 4YG, UK., El Naggar RF; Division of Biomedical and Life Sciences, Faculty of Health and Medicine, Lancaster University, Lancaster LA1 4YG, UK; Department of Virology, Faculty of Veterinary Medicine, University of Sadat City, Sadat 32897, Egypt., Abdelsabour MA; Department of Poultry Viral Vaccines, Veterinary Serum and Vaccine Research Institute (VSVRI), Agriculture Research Centre (ARC), Cairo 11435, Egypt., Madbouly YM; Department of Poultry Viral Vaccines, Veterinary Serum and Vaccine Research Institute (VSVRI), Agriculture Research Centre (ARC), Cairo 11435, Egypt., Atasoy MO; Division of Biomedical and Life Sciences, Faculty of Health and Medicine, Lancaster University, Lancaster LA1 4YG, UK., Ahmed KA; Central Laboratory for Evaluation of Veterinary Biologics, Abbasia, Cairo, 11381, Egypt., El-Safty MM; Department of Pathology, Faculty of Veterinary Medicine, Cairo University, Giza 12211, Egypt., Munir M; Division of Biomedical and Life Sciences, Faculty of Health and Medicine, Lancaster University, Lancaster LA1 4YG, UK. Electronic address: muhammad.munir@lancaster.ac.uk.
Jazyk: angličtina
Zdroj: Microbes and infection [Microbes Infect] 2024 Jan-Feb; Vol. 26 (1-2), pp. 105231. Date of Electronic Publication: 2023 Sep 28.
DOI: 10.1016/j.micinf.2023.105231
Abstrakt: Broad-spectrum antiviral activities of interferon-induced transmembrane proteins (IFITMs) are primarily attributed to in vitro inhibition of viral entry. Here, we used an avian sarcoma-leukosis virus (RCAS)-based gene transfer system and successfully generated chicks that constitutively express chicken IFITM3 (chIFITM3). The chIFITM3-overexpressing chicks showed significant protection and disease tolerance against highly pathogenic avian influenza virus (HPAIV) H5N1 (Clade 2.2.1.2). The chicks, overexpressing chIFITM3, also showed delayed onset of clinical symptoms, reduced viral shedding, and alleviated histopathologic alterations compared to control and challenged chicks. These findings highlight that overexpression of chIFITM3 provide a substantial defense against zoonotic H5N1 in vivo.
(Copyright © 2023 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.)
Databáze: MEDLINE
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