Protocol for assembling and implementing a partially automated system for rearing and handling Anopheles stephensi mosquitoes.

Autor:	Pitre Z; Seattle Children's Research Institute, Seattle, WA 98109, USA; Powerlight Technologies, Kent, WA 98032, USA., Seltzer T; Seattle Children's Research Institute, Seattle, WA 98109, USA., Kalthoff C; Seattle Children's Research Institute, Seattle, WA 98109, USA., Benitez-Cortez M; Seattle Children's Research Institute, Seattle, WA 98109, USA., Yee C; Seattle Children's Research Institute, Seattle, WA 98109, USA., Glennon EKK; Seattle Children's Research Institute, Seattle, WA 98109, USA., Primavera VI; Seattle Children's Research Institute, Seattle, WA 98109, USA., Kaushansky A; Seattle Children's Research Institute, Seattle, WA 98109, USA; Department of Pediatrics, University of Washington, Seattle, WA 98105, USA; Department of Global Health, University of Washington, Seattle, WA 98105, USA. Electronic address: alexis.kaushansky@seattlechildrens.org.
Jazyk:	angličtina
Zdroj:	STAR protocols [STAR Protoc] 2023 Dec 15; Vol. 4 (4), pp. 102621. Date of Electronic Publication: 2023 Sep 28.
DOI:	10.1016/j.xpro.2023.102621
Abstrakt:	Live mosquitoes are required to comprehensively study vector-borne diseases, including transmission. Traditional mosquito-rearing protocols are laborious and time consuming. Here, we present a protocol for assembling and implementing a partially automated system for rearing and handling Anopheles stephensi mosquitoes. We describe steps for assembling a pupation station, self-emptying bucket, pupal funnel and dish vacuum, automatic aspirator, and sugar tubes. We also detail the application of these systems, along with specific limitations. Competing Interests: Declaration of interests The authors declare no competing interests. (Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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