Microphysiological Modeling of Gingival Tissues and Host-Material Interactions Using Gingiva-on-Chip.

Autor: Muniraj G; Faculty of Dentistry, National University of Singapore, Singapore, 119085, Singapore., Tan RHS; Singapore Institute of Manufacturing Technology (SIMTech), Agency for Science, Technology and Research (A*STAR), Singapore, 138634, Singapore., Dai Y; Faculty of Dentistry, National University of Singapore, Singapore, 119085, Singapore., Wu R; Singapore Institute of Manufacturing Technology (SIMTech), Agency for Science, Technology and Research (A*STAR), Singapore, 138634, Singapore., Alberti M; Singapore Institute of Manufacturing Technology (SIMTech), Agency for Science, Technology and Research (A*STAR), Singapore, 138634, Singapore.; REVIVO BioSystems Pte. Ltd., Singapore, 138623, Singapore., Sriram G; Faculty of Dentistry, National University of Singapore, Singapore, 119085, Singapore.; ORCHIDS: Oral Care Health Innovations and Designs Singapore, National University of Singapore, Singapore, 119085, Singapore.; NUS Centre for Additive Manufacturing (AM.NUS), National University of Singapore, Singapore, 117602, Singapore.
Jazyk: angličtina
Zdroj: Advanced healthcare materials [Adv Healthc Mater] 2023 Dec; Vol. 12 (32), pp. e2301472. Date of Electronic Publication: 2023 Oct 13.
DOI: 10.1002/adhm.202301472
Abstrakt: Gingiva plays a crucial barrier role at the interface of teeth, tooth-supporting structures, microbiome, and external agents. To mimic this complex microenvironment, an in vitro microphysiological platform and biofabricated full-thickness gingival equivalents (gingiva-on-chip) within a vertically stacked microfluidic device is developed. This design allowed long-term and air-liquid interface culture, and host-material interactions under flow conditions. Compared to static cultures, dynamic cultures on-chip enabled the biofabrication of gingival equivalents with stable mucosal matrix, improved epithelial morphogenesis, and barrier features. Additionally, a diseased state with disrupted barrier function representative of gingival/oral mucosal ulcers is modeled. The apical flow feature is utilized to emulate the mechanical action of mouth rinse and integrate the assessment of host-material interactions and transmucosal permeation of oral-care formulations in both healthy and diseased states. Although the gingiva-on-chip cultures have thicker and more mature epithelium, the flow of oral-care formulations induced increased tissue disruption and cytotoxic features compared to static conditions. The realistic emulation of mouth rinsing action facilitated a more physiological assessment of mucosal irritation potential. Overall, this microphysiological system enables biofabrication of human gingiva equivalents in intact and ulcerated states, providing a miniaturized and integrated platform for downstream host-material and host-microbiome applications in gingival and oral mucosa research.
(© 2023 The Authors. Advanced Healthcare Materials published by Wiley-VCH GmbH.)
Databáze: MEDLINE
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