Trehalose-6-phosphate signaling regulates lateral root formation in Arabidopsis thaliana.

Autor:	Morales-Herrera S; Department of Plant Biotechnology and Bioinformatics Ghent University, Ghent B-9052, Belgium.; Vlaams Instituut voor Biotechnologie Center for Plant Systems Biology, Ghent B-9052, Belgium.; Laboratory of Molecular Cell Biology, Katholieke Universiteit Leuven, Leuven B3001, Belgium.; Vlaams Instituut voor Biotechnologie-Katholieke Universiteit Leuven Center for Microbiology, Leuven B3001, Belgium., Jourquin J; Department of Plant Biotechnology and Bioinformatics Ghent University, Ghent B-9052, Belgium.; Vlaams Instituut voor Biotechnologie Center for Plant Systems Biology, Ghent B-9052, Belgium., Coppé F; Department of Plant Biotechnology and Bioinformatics Ghent University, Ghent B-9052, Belgium.; Vlaams Instituut voor Biotechnologie Center for Plant Systems Biology, Ghent B-9052, Belgium., Lopez-Galvis L; Department of Plant Biotechnology and Bioinformatics Ghent University, Ghent B-9052, Belgium.; Vlaams Instituut voor Biotechnologie Center for Plant Systems Biology, Ghent B-9052, Belgium.; Laboratory of Molecular Cell Biology, Katholieke Universiteit Leuven, Leuven B3001, Belgium.; Vlaams Instituut voor Biotechnologie-Katholieke Universiteit Leuven Center for Microbiology, Leuven B3001, Belgium., De Smet T; Department of Organic and Macromolecular Chemistry, Laboratory for Organic and Bio-Organic Synthesis, Ghent University, Ghent B-9000, Belgium., Safi A; Department of Plant Biotechnology and Bioinformatics Ghent University, Ghent B-9052, Belgium.; Vlaams Instituut voor Biotechnologie Center for Plant Systems Biology, Ghent B-9052, Belgium., Njo M; Department of Plant Biotechnology and Bioinformatics Ghent University, Ghent B-9052, Belgium.; Vlaams Instituut voor Biotechnologie Center for Plant Systems Biology, Ghent B-9052, Belgium., Griffiths CA; Department of Sustainable Soils and Crops, Rothamsted Research, Harpenden AL5 2JQ, United Kingdom., Sidda JD; Department of Chemistry, Chemistry Research Laboratory, University of Oxford, Oxford OX1 3TA, United Kingdom., Mccullagh JSO; Department of Chemistry, Chemistry Research Laboratory, University of Oxford, Oxford OX1 3TA, United Kingdom., Xue X; Department of Chemistry, Chemistry Research Laboratory, University of Oxford, Oxford OX1 3TA, United Kingdom., Davis BG; Department of Chemistry, Chemistry Research Laboratory, University of Oxford, Oxford OX1 3TA, United Kingdom.; Next Generation Chemistry, The Rosalind Franklin Institute, Didcot OX1 3TA, United Kingdom.; Department of Pharmacology, University of Oxford, Oxford OX1 3TA, United Kingdom., Van der Eycken J; Department of Organic and Macromolecular Chemistry, Laboratory for Organic and Bio-Organic Synthesis, Ghent University, Ghent B-9000, Belgium., Paul MJ; Department of Sustainable Soils and Crops, Rothamsted Research, Harpenden AL5 2JQ, United Kingdom., Van Dijck P; Laboratory of Molecular Cell Biology, Katholieke Universiteit Leuven, Leuven B3001, Belgium.; Vlaams Instituut voor Biotechnologie-Katholieke Universiteit Leuven Center for Microbiology, Leuven B3001, Belgium.; Katholieke Universiteit Leuven Plant Institute, Katholieke Universiteit Leuven, Leuven B3001, Belgium., Beeckman T; Department of Plant Biotechnology and Bioinformatics Ghent University, Ghent B-9052, Belgium.; Vlaams Instituut voor Biotechnologie Center for Plant Systems Biology, Ghent B-9052, Belgium.
Jazyk:	angličtina
Zdroj:	Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2023 Oct 03; Vol. 120 (40), pp. e2302996120. Date of Electronic Publication: 2023 Sep 25.
DOI:	10.1073/pnas.2302996120
Abstrakt:	Plant roots explore the soil for water and nutrients, thereby determining plant fitness and agricultural yield, as well as determining ground substructure, water levels, and global carbon sequestration. The colonization of the soil requires investment of carbon and energy, but how sugar and energy signaling are integrated with root branching is unknown. Here, we show through combined genetic and chemical modulation of signaling pathways that the sugar small-molecule signal, trehalose-6-phosphate (T6P) regulates root branching through master kinases SNF1-related kinase-1 (SnRK1) and Target of Rapamycin (TOR) and with the involvement of the plant hormone auxin. Increase of T6P levels both via genetic targeting in lateral root (LR) founder cells and through light-activated release of the presignaling T6P-precursor reveals that T6P increases root branching through coordinated inhibition of SnRK1 and activation of TOR. Auxin, the master regulator of LR formation, impacts this T6P function by transcriptionally down-regulating the T6P-degrader trehalose phosphate phosphatase B in LR cells. Our results reveal a regulatory energy-balance network for LR formation that links the 'sugar signal' T6P to both SnRK1 and TOR downstream of auxin.
Databáze:	MEDLINE
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