DUSP2 affects bladder cancer prognosis by down-regulating MEK/ERK and P38 MAPK signaling pathways through PTPN7.

Autor: Zou F; Department of Urology, Renmin Hospital of Wuhan University, Wuhan 430060, China., Rao T; Department of Urology, Renmin Hospital of Wuhan University, Wuhan 430060, China., Chen W; Department of Urology, Renmin Hospital of Wuhan University, Wuhan 430060, China., Song T; Department of Urology, Renmin Hospital of Wuhan University, Wuhan 430060, China., Li T; State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, 430026, China., Hu W; Department of Urology, Renmin Hospital of Wuhan University, Wuhan 430060, China., Li L; Department of Urology, Renmin Hospital of Wuhan University, Wuhan 430060, China., Yu W; Department of Urology, Renmin Hospital of Wuhan University, Wuhan 430060, China. Electronic address: ywm.com.cn@163.com., Cheng F; Department of Urology, Renmin Hospital of Wuhan University, Wuhan 430060, China. Electronic address: urology1969@aliyun.com.
Jazyk: angličtina
Zdroj: Cellular signalling [Cell Signal] 2023 Dec; Vol. 112, pp. 110893. Date of Electronic Publication: 2023 Sep 20.
DOI: 10.1016/j.cellsig.2023.110893
Abstrakt: Background: As one of the leading causes of cancer death worldwide, bladder cancer (BCa) ranks 12th in incidence rate. Dual Specific Phosphatase 2 (DUSP2) is a member of the bispecific protein phosphatase subfamily. DUSP2 is closely related to the prognosis of cancer, but the role of DUSP2 in bladder cancer is still unclear. This study aims to explore how DUSP2 affects the prognosis of bladder cancer and clarify the important mechanism in bladder cancer.
Methods: Bioinformatics and experiments have detected the anti-tumor effect of DUSP2. Construct a DUSP2 overexpression cell model, and then use protein blotting experiments to verify the efficiency of transfection. The effects of DUSP2 on proliferation, metastasis, apoptosis, epithelial mesenchymal transition (EMT) and immune invasion of bladder cancer cells were detected in vitro or in vivo. In addition, the mechanism of DUSP2 regulating MEK/ERK through PTPN7 pathway and P38 MAPK inhibiting the progression of bladder cancer was also discussed.
Results: The expression of DUSP2 was down regulated in bladder cancer samples and cell lines. The overexpression of DUSP2 inhibits the proliferation, metastasis and immune microenvironment of bladder cancer cells. In addition, we confirmed that DUSP2 regulates MEK/ERK and P38 MAPK through PTPN7 pathway to inhibit the progression of bladder cancer.
Conclusion: DUSP2 inhibits the progression of bladder cancer by regulating PTPN7. These results suggest that DUSP2/PTPN7/MEK/ERK pathway may become a new therapeutic target for bladder cancer.
Competing Interests: Declaration of Competing Interest All animal experiments were approved by the Animal Experiment Center of Wuhan University. The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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