Validation Procedures for Quantification of Food Allergens by Enzyme-Linked Immunosorbent Assay (ELISA).
| Autor: | Liu C; School of Exercise and Nutritional Sciences, San Diego State University, San Diego, CA, USA. changqi.liu@sdsu.edu., Su M; Fujian Hwa Nan Women's College, Fuzhou, Fujian, China., Champ VD; PerkinElmer Inc., Austin, TX, USA., Gupta S; General Mills, Inc., Minneapolis, MN, USA. |
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| Jazyk: | angličtina |
| Zdroj: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2024; Vol. 2717, pp. 285-304. |
| DOI: | 10.1007/978-1-0716-3453-0_19 |
| Abstrakt: | Enzyme-linked immunosorbent assay (ELISA) is a widely used analytical technique for food allergen detection and quantification. Validating ELISA protocols is important for both assay developers and end users as it ensures method reliability. This chapter describes the protocols for validating the sensitivity, specificity, precision, accuracy, robustness, and ruggedness of an ELISA. Example procedures are also provided for sample preparation, allergen extraction, and ELISA operation. (© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.) |
| Databáze: | MEDLINE |
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