Vegetative insecticidal protein (Vip3A) production by Bacillus thuringiensis Bt294 and its efficacy against Lepidopteran pests ( Spodoptera exigua ).

Autor: Nutaratat P; Biocontrol Technology Research Team, Biorefinery and Bioproduct Technology Research Group, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, 113 Thailand Science Park, Phahonyothin Rd., Khlong Nueng, Khlong Luang, Pathum Thani, 12120, Thailand., Werapan B; Biocontrol Technology Research Team, Biorefinery and Bioproduct Technology Research Group, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, 113 Thailand Science Park, Phahonyothin Rd., Khlong Nueng, Khlong Luang, Pathum Thani, 12120, Thailand., Phosrithong N; Forest entomology and Microbiology Research Group, Forest and Plant Conservation Research Office, Department of National Parks, Wildlife and Plant Conservation, 61 Phahonyothin Rd, Chatuchak, Bangkok, 10900, Thailand., Trakulnaleamsai C; Biocontrol Technology Research Team, Biorefinery and Bioproduct Technology Research Group, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, 113 Thailand Science Park, Phahonyothin Rd., Khlong Nueng, Khlong Luang, Pathum Thani, 12120, Thailand., Rungrod A; Biocontrol Technology Research Team, Biorefinery and Bioproduct Technology Research Group, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, 113 Thailand Science Park, Phahonyothin Rd., Khlong Nueng, Khlong Luang, Pathum Thani, 12120, Thailand., Utamatho M; Biocontrol Technology Research Team, Biorefinery and Bioproduct Technology Research Group, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, 113 Thailand Science Park, Phahonyothin Rd., Khlong Nueng, Khlong Luang, Pathum Thani, 12120, Thailand., Soonsanga S; Biocontrol Technology Research Team, Biorefinery and Bioproduct Technology Research Group, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, 113 Thailand Science Park, Phahonyothin Rd., Khlong Nueng, Khlong Luang, Pathum Thani, 12120, Thailand., Promdonkoy B; Biocontrol Technology Research Team, Biorefinery and Bioproduct Technology Research Group, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, 113 Thailand Science Park, Phahonyothin Rd., Khlong Nueng, Khlong Luang, Pathum Thani, 12120, Thailand., Malairuang K; Biocontrol Technology Research Team, Biorefinery and Bioproduct Technology Research Group, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, 113 Thailand Science Park, Phahonyothin Rd., Khlong Nueng, Khlong Luang, Pathum Thani, 12120, Thailand., Prathumpai W; Biocontrol Technology Research Team, Biorefinery and Bioproduct Technology Research Group, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, 113 Thailand Science Park, Phahonyothin Rd., Khlong Nueng, Khlong Luang, Pathum Thani, 12120, Thailand.
Jazyk: angličtina
Zdroj: Biotechnology reports (Amsterdam, Netherlands) [Biotechnol Rep (Amst)] 2023 Sep 12; Vol. 40, pp. e00812. Date of Electronic Publication: 2023 Sep 12 (Print Publication: 2023).
DOI: 10.1016/j.btre.2023.e00812
Abstrakt: A vegetative insecticidal protein, Vip3A, is highly active against lepidopteran pests, which are the most important pests in most tropical countries. An important aspect of the successful commercial production of this bacterial insecticide is the development of bacterial culture media that maximize the titres of this protein and cost reduction. This study aimed to investigate and optimize Vip3A production by Bacillus thuringiensis Bt294 using statistical methods and 3-step sequential approaches. The experimental design showed that the production of Vip3A was maximized to 300 mg/L when the bacterium was cultivated in medium composed of 5.05 g/L glycerol, 49.17 g/L soytone, 30.05 g/L casein hydrolysate, 1.99 g/L CaCl 2 .2H 2 O, 7.5 mg/L CuSO4, 15 mg/L MnSO 4 .H 2 O, 9.4 g/L K 2 HPO 4 , 2.2 g/L KH 2 PO 4 , 0.2 g/L MgSO 4 .7H 2 O, 5 g/L yeast extract, 2.5 mg/L NiCl 2 .6H2O and 3 mL/L vitamin solution. B. thuringiensis Bt294 Vip3A toxin was highly toxic to Spodoptera exigua with LC50 values of 187.1 ng/cm 2 at 7 days. This result demonstrated that a high titre of Vip3A produced by B. thuringiensis Bt294 will be useful as a biological control agent. This optimization will allow production to be scaled up for commercial production in the future.
Competing Interests: The authors declare no conflicts of interest. Experimental investigation, methodology, data curation, formal analysis and writing-original draft preparation were done by Pumin Nutaratat, investigation and methodology were done by Borworn Werapan, Netnapa Phosrithong, Chutchanun Trakulnaleamsai, Amporn Rungrod and Kwanruthai Malairuang, supervision was done by Mongkon Utamatho, Sumarin Soonsanga, Boonhiang Promdonkoy, and conceptualization, funding acquisition, project administration, resources, supervision, writing-review and editing was accomplished by Wai Prathumpai. The funding source of the research work is already stated in the manuscript and there is no financial/personal interest that affected the objectives of the studies. All authors have read and agreed to the published version of the manuscript.
(© 2023 The Authors. Published by Elsevier B.V.)
Databáze: MEDLINE
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