Linking bacterial tetrabromopyrrole biosynthesis to coral metamorphosis.

Autor: Alker AT; Department of Biology and Viral Information Institute, San Diego State University, San Diego, CA, 92182, USA., Farrell MV; Department of Biology and Viral Information Institute, San Diego State University, San Diego, CA, 92182, USA., Demko AM; Smithsonian Marine Station, Ft. Pierce, FL, 34949, USA., Purdy TN; Center for Marine Biotechnology and Biomedicine, Scripps Institution of Oceanography, University of California, San Diego, La Jolla, CA, 92093, USA., Adak S; Center for Marine Biotechnology and Biomedicine, Scripps Institution of Oceanography, University of California, San Diego, La Jolla, CA, 92093, USA., Moore BS; Center for Marine Biotechnology and Biomedicine, Scripps Institution of Oceanography, University of California, San Diego, La Jolla, CA, 92093, USA., Sneed JM; Smithsonian Marine Station, Ft. Pierce, FL, 34949, USA., Paul VJ; Smithsonian Marine Station, Ft. Pierce, FL, 34949, USA., Shikuma NJ; Department of Biology and Viral Information Institute, San Diego State University, San Diego, CA, 92182, USA. nshikuma@sdsu.edu.
Jazyk: angličtina
Zdroj: ISME communications [ISME Commun] 2023 Sep 19; Vol. 3 (1), pp. 98. Date of Electronic Publication: 2023 Sep 19.
DOI: 10.1038/s43705-023-00309-6
Abstrakt: An important factor dictating coral fitness is the quality of bacteria associated with corals and coral reefs. One way that bacteria benefit corals is by stimulating the larval to juvenile life cycle transition of settlement and metamorphosis. Tetrabromopyrrole (TBP) is a small molecule produced by bacteria that stimulates metamorphosis with and without attachment in a range of coral species. A standing debate remains, however, about whether TBP biosynthesis from live Pseudoalteromonas bacteria is the primary stimulant of coral metamorphosis. In this study, we create a Pseudoalteromonas sp. PS5 mutant lacking the TBP brominase gene, bmp2. Using this mutant, we confirm that the bmp2 gene is critical for TBP biosynthesis in Pseudoalteromonas sp. PS5. Mutation of this gene ablates the bacterium's ability in live cultures to stimulate the metamorphosis of the stony coral Porites astreoides. We further demonstrate that expression of TBP biosynthesis genes is strongest in stationary and biofilm modes of growth, where Pseudoalteromonas sp. PS5 might exist within surface-attached biofilms on the sea floor. Finally, we create a modular transposon plasmid for genomic integration and fluorescent labeling of Pseudoalteromonas sp. PS5 cells. Our results functionally link a TBP biosynthesis gene from live bacteria to a morphogenic effect in corals. The genetic techniques established here provide new tools to explore coral-bacteria interactions and could help to inform future decisions about utilizing marine bacteria or their products for coral restoration.
(© 2023. ISME Publications B.V.)
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje