Co-immobilization of galactose oxidase, catalase, and Mn-superoxide dismutase for efficient conversion of 5-hydroxymethylfurfural to 2,5-diformylfuran in water.

Autor: Yang L; Department of Biological Engineering, Beijing University of Chemical Technology, Beijing, China., Wei J; Department of Biological Engineering, Beijing University of Chemical Technology, Beijing, China., Feng W; Department of Biological Engineering, Beijing University of Chemical Technology, Beijing, China. Electronic address: fengwei@mail.buct.edu.cn.
Jazyk: angličtina
Zdroj: Colloids and surfaces. B, Biointerfaces [Colloids Surf B Biointerfaces] 2023 Nov; Vol. 231, pp. 113541. Date of Electronic Publication: 2023 Sep 13.
DOI: 10.1016/j.colsurfb.2023.113541
Abstrakt: The three enzymes galactose oxidase (GO), catalase (CAT), and Mn-superoxide dismutase (SOD) were simultaneously immobilized by coordinating to Cu II in phosphate buffer saline. The biocatalyst GO&CAT&SOD@Cu II was used for the conversion of 5-hydroxymethylfurfural (HMF). The immobilized GO catalyzes the oxidation of HMF to 2,5-diformylfuran (DFF), concomitantly the co-substrate O 2 is reduced to hydrogen peroxide (H 2 O 2 ). A portion of the byproduct H 2 O 2 is broken down to O 2 and H 2 O by the co-immobilized CAT, and the evolved O 2 can be recycled and used as the co-substrate. A portion of the byproduct H 2 O 2 is broken down to produce hydroxyl radicals •OH under the synergistic catalysis of the immobilized SOD and coordinated Cu II , and the produced •OH can reactivate the immobilized galactose oxidase. Two aspects contribute to the high catalytic efficiency by GO&CAT&SOD@Cu II : the reactivation of the immobilized galactose oxidase by producing •OH and the enrichment of the co-substate O 2 by recycling the produced O 2 . For the conversion of 10 mM HMF, GO&CAT&SOD@Cu II (with encapsulated GO 0.2 mg/mL) achieved 97% HMF conversion within 2 h reaction. In contrast, free galactose oxidase M 3-5 variant (ACS Catalysis 2018, 8, 4025) (0.2 mg/mL) achieved 25.3% HMF conversion within 2 h reaction. All the reactions were carried out in pure water, not in PBS.
Competing Interests: Declaration of Competing Interest The authors report no declarations of interest.
(Copyright © 2023 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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