Heterogeneity of mesenchymal cells in human amniotic membrane at term.

Autor: Cortes-Sandoval S; Department of Obstetrics and Gynecology, Virgen de la Arrixaca Hospital, IMIB, Murcia, Spain.; Department of Cell Biology and Histology, IMIB, School of Medicine, Regional Campus of International Excellence 'Campus Mare Nostrum', University of Murcia, Murcia, Spain., Seco-Rovira V; Department of Cell Biology and Histology, IMIB, School of Medicine, Regional Campus of International Excellence 'Campus Mare Nostrum', University of Murcia, Murcia, Spain., Beltrán-Frutos E; Department of Cell Biology and Histology, IMIB, School of Medicine, Regional Campus of International Excellence 'Campus Mare Nostrum', University of Murcia, Murcia, Spain., Serrano-Sánchez MI; Department of Cell Biology and Histology, IMIB, School of Medicine, Regional Campus of International Excellence 'Campus Mare Nostrum', University of Murcia, Murcia, Spain., Martínez-Hernández J; Department of Cell Biology and Histology, IMIB, School of Medicine, Regional Campus of International Excellence 'Campus Mare Nostrum', University of Murcia, Murcia, Spain., Ferrer C; Department of Cell Biology and Histology, IMIB, School of Medicine, Regional Campus of International Excellence 'Campus Mare Nostrum', University of Murcia, Murcia, Spain., Delgado JL; Department of Obstetrics and Gynecology, Virgen de la Arrixaca Hospital, IMIB, Murcia, Spain., Insausti CL; Hematology Service, Virgen de la Arrixaca University Hospital, IMIB, Murcia, Spain., Blanquer M; Hematology Service, Virgen de la Arrixaca University Hospital, IMIB, Murcia, Spain., Pastor LM; Department of Cell Biology and Histology, IMIB, School of Medicine, Regional Campus of International Excellence 'Campus Mare Nostrum', University of Murcia, Murcia, Spain. bioetica@um.es.
Jazyk: angličtina
Zdroj: Histology and histopathology [Histol Histopathol] 2024 May; Vol. 39 (5), pp. 573-593. Date of Electronic Publication: 2023 Sep 04.
DOI: 10.14670/HH-18-660
Abstrakt: There is increasing interest in understanding the tissue biology of human amniotic membrane (hAM) given its applications in medicine. One cellular component is mesenchymal cells, which can be extracted, cultured and differentiated " in vitro " into various cell types. These studies show that there is heterogeneity among mesenchymal cells. The aim of this work is to study the membrane in situ to determine whether this cellular heterogeneity exists. The hAMs were obtained from caesarean deliveries at term and analyzed by histological techniques. Types I-III mesenchymal cells and Hofbauer were distinguished by light microscopy. Histochemically, mesenchymal cell types showed successively increasing positivity to: PAS, vimentin, fibronectin, and Concanavalin-A; VGEF, TGF-β2, PDGF-C, FGF-2. By the semiquantitative point of view, the percentage of Type II cells was 60%, significantly higher than the other types. With transmission electron microscopy, an intermediate cell type between II-III was observed. Strong vesiculation of the rough endoplasmic reticulum (RER) with exocytosis was observed. In addition, an accumulation of a similar material to the extracellular matrix in the RER caused its dilation especially in type III TEM cells. Some of this material acquired a globular structure. These structures were also found free in the extracellular matrix. In conclusion, the mesenchymal cells of the fibroblastic layer of the hAMs studied are heterogeneous, with some undifferentiated and others with a probably senescent fibroblastic phenotype with accumulation in their RER of fibronectin. These results may be of interest to extract mesenchymal cells from hAMs for use in regenerative medicine and to better understand the mechanisms of fetal membrane rupture.
(©The Author(s) 2024. Open Access. This article is licensed under a Creative Commons CC-BY International License.)
Databáze: MEDLINE
Externí odkaz: