Behaviour of hTERT in the tears of neophyte contact lens wearers during the sleep/wake cycle.

Autor: Alotaibi S; School of Optometry & Vision Science, University of New South Wales, Sydney, Australia; Department of Optometry & Vision Science, College of Applied Medical Science, King Saud University, Riyadh, Saudi Arabia. Electronic address: salotabi1@ksu.edu.sa., Papas E; School of Optometry & Vision Science, University of New South Wales, Sydney, Australia., Ozkan J; School of Optometry & Vision Science, University of New South Wales, Sydney, Australia., Misra SL; Department of Ophthalmology, New Zealand National Eye Centre, Faculty of Medical and Health Sciences, The University of Auckland, New Zealand., Markoulli M; School of Optometry & Vision Science, University of New South Wales, Sydney, Australia.
Jazyk: angličtina
Zdroj: Contact lens & anterior eye : the journal of the British Contact Lens Association [Cont Lens Anterior Eye] 2023 Dec; Vol. 46 (6), pp. 102060. Date of Electronic Publication: 2023 Sep 14.
DOI: 10.1016/j.clae.2023.102060
Abstrakt: Purpose: To investigate the behaviour of telomerase reverse transcriptase (hTERT) in the tears of healthy neophyte contact lenses-wearing individuals during the sleep/wake cycle. A subsequent aim was to investigate whether hTERT behaviour was associated with inflammatory mediators interleukin-6 (IL-6) and tumour necrosis factor-alpha (TNF-α) in tears.
Methods: Flush tears were collected from 19 healthy, non-contact lens-wearing participants (11 males, 8 females, mean age 31.9 ± 5.7 years), before and during contact lens wear. Tears were collected at noon, before sleep and upon awakening and levels of hTERT, IL-6 and TNF-α, were determined using enzyme-linked immunosorbent assays (ELISA).
Results: hTERT levels (median [interquartile range]) during contact lens wear were significantly higher before sleep (436.5 (263.9 - 697.7) ng/ml compared to the same time point without contact lenses (256.1 (0.0 - 590.9) ng/ml (p = 0.01). There was no difference between contact lens wear (851.3 [353.2 - 2109.9]) ng/ml, and no wear (1091.0 [492.3 - 3045.4]) ng/ml, upon awakening (p = 0.94). A significant increase was found upon awakening compared to before sleep, irrespective of the presence of a contact lens (p = 0.02). IL-6 and TNF-α levels in tears were below the limit of detection.
Conclusions: The study showed that hTERT increases after a contact lens is placed on the eye, but this change is small, compared to the impact of overnight eye closure. Taken together with the lack of responses of the inflammatory markers monitored at the same time points, this may suggest that hTERT can respond both to low-level stress stimuli acting on the ocular surface, and to situations where inflammation is a likely factor.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Crown Copyright © 2023. Published by Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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