Identification and functional characterization of REGULATORY PARTICLE NON-ATPASE 1a-like (AhRPN1a-like) in peanuts during aluminum-induced programmed cell death.

Autor: Liao G; College of Agriculture, Guangxi University, Nanning, Guangxi, China., Luo S; College of Agriculture, Guangxi University, Nanning, Guangxi, China., Li X; College of Agriculture, Guangxi University, Nanning, Guangxi, China., Li A; College of Agriculture, Guangxi University, Nanning, Guangxi, China., Mo Y; College of Agriculture, Guangxi University, Nanning, Guangxi, China., Wang A; College of Agriculture, Guangxi University, Nanning, Guangxi, China; Key Laboratory of Crop Cultivation and Physiology, Education Department of Guangxi Zhuang Autonomous Region, Guangxi University, Nanning, 530004, China; Guangxi Key Laboratory of Agro-environment and Agric-products Safety, College of Agriculture, Guangxi University, Nanning, Guangxi, China., Xiao D; College of Agriculture, Guangxi University, Nanning, Guangxi, China; Key Laboratory of Crop Cultivation and Physiology, Education Department of Guangxi Zhuang Autonomous Region, Guangxi University, Nanning, 530004, China; Guangxi Key Laboratory of Agro-environment and Agric-products Safety, College of Agriculture, Guangxi University, Nanning, Guangxi, China., He LF; College of Agriculture, Guangxi University, Nanning, Guangxi, China; Key Laboratory of Crop Cultivation and Physiology, Education Department of Guangxi Zhuang Autonomous Region, Guangxi University, Nanning, 530004, China; Guangxi Key Laboratory of Agro-environment and Agric-products Safety, College of Agriculture, Guangxi University, Nanning, Guangxi, China., Zhan J; College of Agriculture, Guangxi University, Nanning, Guangxi, China; Key Laboratory of Crop Cultivation and Physiology, Education Department of Guangxi Zhuang Autonomous Region, Guangxi University, Nanning, 530004, China; Guangxi Key Laboratory of Agro-environment and Agric-products Safety, College of Agriculture, Guangxi University, Nanning, Guangxi, China. Electronic address: may2399@163.com.
Jazyk: angličtina
Zdroj: Journal of plant physiology [J Plant Physiol] 2023 Oct; Vol. 289, pp. 154079. Date of Electronic Publication: 2023 Aug 30.
DOI: 10.1016/j.jplph.2023.154079
Abstrakt: The toxicity of aluminum (Al) in acidic soil is a prevalent problem and causes reduced crop yields. In the plant response to Al toxicity, programmed cell death (PCD) appears to be one of the important mechanisms. However, the regulation of Al-induced PCD remains poorly understood. Here, we found that an uncharacterized protein REGULATORY PARTICLE NON-ATPASE 1a-like in peanut (AhRPN1a-like), located in the nucleus and cytoplasm, directly interacted with type I metacaspase in peanut (AhMC1). The overexpression of AhRPN1a-like in Arabidopsis strongly enhanced Al inhibition of root growth with a loss of root tip cell viability. Furthermore, in response to Al treatment, the VIGS knockdown line of AhRPN1a-like in peanut displayed decreased transcription of AhMC1, increased root growth, reduced Al-induced PCD and decreased 26S proteasomal activity. Taken together, these findings demonstrated that AhRPN1a-like interacted directly with AhMC1, and promotes the occurrence of Al-induced PCD via the 26S proteasome pathway, thereby reducing Al-resistance.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier GmbH. All rights reserved.)
Databáze: MEDLINE
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