Differential metabolic reprogramming in developing soybean embryos in response to nutritional conditions and abscisic acid.

Autor: Pavlovic T; Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI-CONICET), Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Suipacha 570, S2000LRJ, Rosario, Santa Fe, Argentina., Margarit E; Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI-CONICET), Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Suipacha 570, S2000LRJ, Rosario, Santa Fe, Argentina., Müller GL; Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI-CONICET), Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Suipacha 570, S2000LRJ, Rosario, Santa Fe, Argentina., Saenz E; Instituto de Investigaciones en Ciencias Agrarias de Rosario (IICAR-CONICET), Facultad de Ciencias Agrarias, Universidad Nacional de Rosario, Campo Experimental Villarino CC14, S2125ZAA, Zavalla, Santa Fe, Argentina., Ruzzo AI; Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI-CONICET), Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Suipacha 570, S2000LRJ, Rosario, Santa Fe, Argentina., Drincovich MF; Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI-CONICET), Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Suipacha 570, S2000LRJ, Rosario, Santa Fe, Argentina., Borrás L; Instituto de Investigaciones en Ciencias Agrarias de Rosario (IICAR-CONICET), Facultad de Ciencias Agrarias, Universidad Nacional de Rosario, Campo Experimental Villarino CC14, S2125ZAA, Zavalla, Santa Fe, Argentina., Saigo M; Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI-CONICET), Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Suipacha 570, S2000LRJ, Rosario, Santa Fe, Argentina. saigo@cefobi-conicet.gov.ar., Wheeler MCG; Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI-CONICET), Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Suipacha 570, S2000LRJ, Rosario, Santa Fe, Argentina. gerrard@cefobi-conicet.gov.ar.
Jazyk: angličtina
Zdroj: Plant molecular biology [Plant Mol Biol] 2023 Oct; Vol. 113 (1-3), pp. 89-103. Date of Electronic Publication: 2023 Sep 13.
DOI: 10.1007/s11103-023-01377-x
Abstrakt: Seed storage compound deposition is influenced by both maternal and filial tissues. Within this framework, we analyzed strategies that operate during the development and filling of soybean embryos, using in vitro culture systems combined with metabolomics and proteomics approaches. The carbon:nitrogen ratio (C:N) of the maternal supply and the hormone abscisic acid (ABA) are specific and interacting signals inducing differential metabolic reprogrammings linked to changes in the accumulation of storage macromolecules like proteins or oils. Differences in the abundance of sugars, amino acids, enzymes, transporters, transcription factors, and proteins involved in signaling were detected. Embryos adapted to the nutritional status by enhancing the metabolism of both carbon and nitrogen under lower C:N ratio condition or only carbon under higher C:N ratio condition. ABA turned off multiple pathways especially in high availability of amino acids, prioritizing the storage compounds biosynthesis. Common responses induced by ABA involved increased sucrose uptake (to increase the sink force) and oleosin (oil body structural component) accumulation. In turn, ABA differentially promoted protein degradation under lower nitrogen supply in order to sustain the metabolic demands. Further, the operation of a citrate shuttle was suggested by transcript quantification and enzymatic activity measurements. The results obtained are useful to help define biotechnological tools and technological approaches to improve oil and protein yields, with direct impact on human and animal nutrition as well as in green chemistry.
(© 2023. The Author(s), under exclusive licence to Springer Nature B.V.)
Databáze: MEDLINE
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