Effects of cyclic adenosine monophosphate modulating agents during oocyte pre-maturation and the role of melatonin on in vitro maturation of bovine cumulus-oocyte complexes.

Autor: Silva BR; Laboratory of Biotechnology and Physiology of Reproduction (LABIREP), Federal University of Ceara, Av. Maurocélio Rocha Ponte 100, Sobral 62041-040, Ceará, Brazil., Barrozo LG; Laboratory of Biotechnology and Physiology of Reproduction (LABIREP), Federal University of Ceara, Av. Maurocélio Rocha Ponte 100, Sobral 62041-040, Ceará, Brazil., Nascimento DR; Laboratory of Biotechnology and Physiology of Reproduction (LABIREP), Federal University of Ceara, Av. Maurocélio Rocha Ponte 100, Sobral 62041-040, Ceará, Brazil., Costa FC; Laboratory of Biotechnology and Physiology of Reproduction (LABIREP), Federal University of Ceara, Av. Maurocélio Rocha Ponte 100, Sobral 62041-040, Ceará, Brazil., Azevedo VAN; Laboratory of Biotechnology and Physiology of Reproduction (LABIREP), Federal University of Ceara, Av. Maurocélio Rocha Ponte 100, Sobral 62041-040, Ceará, Brazil., Paulino LRFM; Laboratory of Biotechnology and Physiology of Reproduction (LABIREP), Federal University of Ceara, Av. Maurocélio Rocha Ponte 100, Sobral 62041-040, Ceará, Brazil., Lopes EPF; Faculty of Veterinary Medicine, Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), State University of Ceará, Fortaleza, Ceará, Brazil., Batista ALPS; Laboratory of Biotechnology and Physiology of Reproduction (LABIREP), Federal University of Ceara, Av. Maurocélio Rocha Ponte 100, Sobral 62041-040, Ceará, Brazil., Aguiar FLN; Department of Veterinary Medicine, Sousa Campus, Federal Institute of Education, Science and Technology of Paraíba, Sousa, Paraíba, Brazil., Peixoto CA; Laboratory of Ultrastructure, CPqAM/FIOCRUZ, Federal University of Pernambuco, Recife, Pernambuco, Brazil., Donato MAM; Laboratory of Ultrastructure, CPqAM/FIOCRUZ, Federal University of Pernambuco, Recife, Pernambuco, Brazil., Rodrigues APR; Faculty of Veterinary Medicine, Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), State University of Ceará, Fortaleza, Ceará, Brazil., Silva JRV; Laboratory of Biotechnology and Physiology of Reproduction (LABIREP), Federal University of Ceara, Av. Maurocélio Rocha Ponte 100, Sobral 62041-040, Ceará, Brazil. Electronic address: jrvsilva@ufc.br.
Jazyk: angličtina
Zdroj: Animal reproduction science [Anim Reprod Sci] 2023 Oct; Vol. 257, pp. 107327. Date of Electronic Publication: 2023 Sep 09.
DOI: 10.1016/j.anireprosci.2023.107327
Abstrakt: This study investigated the effects of cyclic adenosine monophosphate modulating during cumulus-oocyte complexes (COCs) pre-maturation and the role of melatonin on in vitro maturation (IVM) of bovine COCs. In experiment one, COCs were pre-matured for 8 h in control medium or with 3-isobutyl-1-methylxanthine (IBMX) and forskolin, IBMX and C-type natriuretic peptide, c-type natriuretic peptide and forskolin or IBMX, forskolin and c-type natriuretic peptide. Then, meiotic progression was evaluated. In experiment two, COCs were pre-matured, followed by IVM in control medium alone or with 10 -6 , 10 -7 or 10 -8 M melatonin. After IVM, chromatin configuration, transzonal projections (TZPs), reactive oxygen species, mitochondrial distribution, ultrastructure and mRNA expression for antioxidant enzymes were evaluated. In experiment 1, COCs pre-matured with both C-type natriuretic peptide and forskolin or C-type natriuretic peptide, forskolin and IBMX had lower meiotic resumption rate when compared to control. Considering that IBMX had not an additional effect to potentiate inhibition of meiotic resumption, a combination of C-type natriuretic peptide and forskolin was chosen. In experiment 2, COCs matured with 10 -8 M melatonin had greater rates of meiotic resumption when compared to the other treatments (P < 0.05). The COCs matured with 10 -7 or 10 -8 M melatonin had greater mitochondrial activity (P < 0.05), while those matured with 10 -6 or 10 -8 M of melatonin had greater levels of TZPs. Ultrastructure of oocyte and cumulus cells after IVM with melatonin was relatively well preserved. COCs matured with 10 -8 M melatonin increased mRNA expression for superoxide dismutase (SOD) and catalase (CAT) (P < 0.05), when compared to non-cultured and pre-matured COCs, respectively. In conclusion, bovine COC pre-maturation with C-type natriuretic peptide and forskolin, followed by IVM with 10 -8 M melatonin improves meiotic resumption rates, TZPs, mitochondrial distribution and mRNA expression for SOD and CAT.
Competing Interests: Declaration of Competing Interest The authors declare that they have no competing interests.
(Copyright © 2023 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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