Distribution of importin-α isoforms in poultry species and their tissue- and age-related differences.

Autor: Herbst A; Clinic for Poultry, University of Veterinary Medicine Hannover, Foundation, Buenteweg 17, 30559 Hannover, Germany. Electronic address: alexandra.herbst@tiho-hannover.de., Bexter F; Clinic for Poultry, University of Veterinary Medicine Hannover, Foundation, Buenteweg 17, 30559 Hannover, Germany. Electronic address: frederik.bexter@tiho-hannover.de., Kouassi NM; Leibniz-Institute for Virology, Martinistraße 52, 20251 Hamburg, Germany. Electronic address: nancy.mounogou@leibniz-liv.de., Gabriel G; Leibniz-Institute for Virology, Martinistraße 52, 20251 Hamburg, Germany; Institute for Virology, University of Veterinary Medicine Hannover, Foundation, Buenteweg 17, 30559 Hannover, Germany. Electronic address: guelsah.gabriel@leibniz-liv.de., Rautenschlein S; Clinic for Poultry, University of Veterinary Medicine Hannover, Foundation, Buenteweg 17, 30559 Hannover, Germany. Electronic address: silke.rautenschlein@tiho-hannover.de.
Jazyk: angličtina
Zdroj: Research in veterinary science [Res Vet Sci] 2023 Nov; Vol. 164, pp. 104994. Date of Electronic Publication: 2023 Aug 30.
DOI: 10.1016/j.rvsc.2023.104994
Abstrakt: While importin-α is well studied in mammals, the knowledge in avian species is still limited. In this study, we compared the mRNA expression patterns of five importin-α isoforms in the respiratory tract, liver, and spleen of chickens, turkeys, and pekin ducks in two different age-groups. In addition, we determined the distribution of importin-α in selected tissue of conchae, trachea, and lung of post-hatch chickens at all cellular levels by immunohistochemical staining. Our results indicate that importin-α3 is the most abundant isoform in the respiratory tract of chickens, turkeys, and pekin ducks. Moreover, importin-α is expressed as a gradient with lowest mRNA levels in the conchae and highest levels in the lung. The mRNA expression levels of most isoforms were higher in tissues from post-hatch chickens and turkeys in comparison to the corresponding embryos. In contrast to that, duck embryos mostly show higher mRNA expression levels of importin-α than post-hatch ducks.
Competing Interests: Declaration of Competing Interest The authors declare no conflict of interest.
(Copyright © 2023 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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