| Autor: |
Carvalho JVG; Laboratory of Wild Animal Biotechnology and Medicine, Faculty of Veterinary Medicine, Federal University of Pará, Castanhal 68740-970, Brazil.; Postgraduate Program in Animal Health and Production in the Amazon, Federal Rural University of the Amazon, Belém 66077-830, Brazil., Soares ARB; Laboratory of Wild Animal Biotechnology and Medicine, Faculty of Veterinary Medicine, Federal University of Pará, Castanhal 68740-970, Brazil.; Postgraduate Program of Animal Reproduction in Amazon, Institute of Veterinary Medicine, Federal University of Pará, Castanhal 68740-970, Brazil., Leão DL; Laboratory of Wild Animal Biotechnology and Medicine, Faculty of Veterinary Medicine, Federal University of Pará, Castanhal 68740-970, Brazil., Reis AN; Laboratory of Wild Animal Biotechnology and Medicine, Faculty of Veterinary Medicine, Federal University of Pará, Castanhal 68740-970, Brazil., Santos RR; Laboratory of Wild Animal Biotechnology and Medicine, Faculty of Veterinary Medicine, Federal University of Pará, Castanhal 68740-970, Brazil., Rodrigues APR; Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), State University of Ceara, Fortaleza 60714-903, Brazil., Domingues SFS; Laboratory of Wild Animal Biotechnology and Medicine, Faculty of Veterinary Medicine, Federal University of Pará, Castanhal 68740-970, Brazil.; Postgraduate Program in Animal Health and Production in the Amazon, Federal Rural University of the Amazon, Belém 66077-830, Brazil.; Postgraduate Program of Animal Reproduction in Amazon, Institute of Veterinary Medicine, Federal University of Pará, Castanhal 68740-970, Brazil. |
| Abstrakt: |
Vitrification is essential for successful tissue cryopreservation and biobanking in wild cats. This study aimed to compare different methods of vitrification (Ovarian Tissue Cryosystem-OTC, Straws-STW, and Solid Surface vitrification-SSV) for testicular fragment vitrification in tom cats. Testicular fragments were recovered from five adult tom cats and subjected to equilibrium vitrification using different cryovials and methods under the same conditions of vitrification solutions and cryoprotectants. The efficiencies of the methods were evaluated using histological analysis of spermatogonia and Sertoli cell nuclei, seminiferous tubular basement membrane detachment, and the gonadal epithelium shrinkage score scale. Cell viability was assessed using Hoechst PI and Terminal deoxynucleotidyl transferase nick end labeling (TUNEL) assay. The results showed that OTC is an effective vitrification method for maintaining the distinction between spermatogonia and Sertoli cells. OTC was similar to the control for basal membrane detachment parameters ( p = 0.05). Epithelial shrinkage was low in the SSV group, which showed the highest percentage of viable cells among the vitrified groups ( p = 0.0023). The OTC and SSV vitrification methods were statistically similar in terms of the percentage of TUNEL-positive cells ( p = 0.05). Therefore, OTC and SSV provide favorable conditions for maintaining viable cat testicular tissue cells after vitrification. |
