Genetic manipulation of Patescibacteria provides mechanistic insights into microbial dark matter and the epibiotic lifestyle.

Autor:	Wang Y; Department of Microbiology, University of Washington, Seattle, WA 98109, USA., Gallagher LA; Department of Microbiology, University of Washington, Seattle, WA 98109, USA., Andrade PA; Department of Microbiology, University of Washington, Seattle, WA 98109, USA., Liu A; Department of Microbiology, University of Washington, Seattle, WA 98109, USA., Humphreys IR; Department of Biochemistry, University of Washington, Seattle, WA 98195, USA; Institute for Protein Design, University of Washington, Seattle, WA 98195, USA., Turkarslan S; Institute for Systems Biology, Seattle, WA 98109, USA., Cutler KJ; Department of Physics, University of Washington, Seattle, WA 98195, USA., Arrieta-Ortiz ML; Institute for Systems Biology, Seattle, WA 98109, USA., Li Y; Department of Microbiology, University of Washington, Seattle, WA 98109, USA; Institute for Systems Biology, Seattle, WA 98109, USA., Radey MC; Department of Microbiology, University of Washington, Seattle, WA 98109, USA., McLean JS; Department of Microbiology, University of Washington, Seattle, WA 98109, USA; Department of Periodontics, University of Washington, Seattle, WA 98195, USA., Cong Q; Eugene McDermott Center for Human Growth and Development, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA; Department of Biophysics, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA; Harold C. Simmons Comprehensive Cancer Center, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA., Baker D; Department of Biochemistry, University of Washington, Seattle, WA 98195, USA; Institute for Protein Design, University of Washington, Seattle, WA 98195, USA; Howard Hughes Medical Institute, University of Washington, Seattle, WA 98109, USA., Baliga NS; Institute for Systems Biology, Seattle, WA 98109, USA., Peterson SB; Department of Microbiology, University of Washington, Seattle, WA 98109, USA., Mougous JD; Department of Microbiology, University of Washington, Seattle, WA 98109, USA; Howard Hughes Medical Institute, University of Washington, Seattle, WA 98109, USA; Microbial Interactions and Microbiome Center, University of Washington, Seattle, WA 98195, USA. Electronic address: mougous@uw.edu.
Jazyk:	angličtina
Zdroj:	Cell [Cell] 2023 Oct 26; Vol. 186 (22), pp. 4803-4817.e13. Date of Electronic Publication: 2023 Sep 07.
DOI:	10.1016/j.cell.2023.08.017
Abstrakt:	Patescibacteria, also known as the candidate phyla radiation (CPR), are a diverse group of bacteria that constitute a disproportionately large fraction of microbial dark matter. Its few cultivated members, belonging mostly to Saccharibacteria, grow as epibionts on host Actinobacteria. Due to a lack of suitable tools, the genetic basis of this lifestyle and other unique features of Patescibacteira remain unexplored. Here, we show that Saccharibacteria exhibit natural competence, and we exploit this property for their genetic manipulation. Imaging of fluorescent protein-labeled Saccharibacteria provides high spatiotemporal resolution of phenomena accompanying epibiotic growth, and a transposon-insertion sequencing (Tn-seq) genome-wide screen reveals the contribution of enigmatic Saccharibacterial genes to growth on their hosts. Finally, we leverage metagenomic data to provide cutting-edge protein structure-based bioinformatic resources that support the strain Southlakia epibionticum and its corresponding host, Actinomyces israelii, as a model system for unlocking the molecular underpinnings of the epibiotic lifestyle. Competing Interests: Declaration of interests The authors declare no competing interests. (Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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