Investigating the impact of inbuilt cold atmospheric pressure plasma on molecular assemblies of tryptophan enantiomers: in vitro fabrication of self-assembled supramolecular structures.

Autor:	Basumatary D; Institute of Advanced Study in Science and Technology, (An Autonomous Institute Under DST, Govt. of India) Vigyan Path, Paschim Boragaon, Garchuk Guwahati 781035 Assam India kamatchi.sankaran@gmail.com.; Academy of Scientific and Innovative Research (AcSIR) Ghaziabad 201002 India., Bailung H; Institute of Advanced Study in Science and Technology, (An Autonomous Institute Under DST, Govt. of India) Vigyan Path, Paschim Boragaon, Garchuk Guwahati 781035 Assam India kamatchi.sankaran@gmail.com., Jorvekar SB; Department of Pharmaceutical Analysis, National Institute of Pharmaceutical Education and Research (NIPER) Sila Katamur (Halugurisuk), PO: Changsari Kamrup Assam 781101 India., Borkar RM; Department of Pharmaceutical Analysis, National Institute of Pharmaceutical Education and Research (NIPER) Sila Katamur (Halugurisuk), PO: Changsari Kamrup Assam 781101 India., Sankaranarayanan K; Institute of Advanced Study in Science and Technology, (An Autonomous Institute Under DST, Govt. of India) Vigyan Path, Paschim Boragaon, Garchuk Guwahati 781035 Assam India kamatchi.sankaran@gmail.com.
Jazyk:	angličtina
Zdroj:	RSC advances [RSC Adv] 2023 Sep 06; Vol. 13 (38), pp. 26640-26649. Date of Electronic Publication: 2023 Sep 06 (Print Publication: 2023).
DOI:	10.1039/d3ra04086k
Abstrakt:	The advancements in understanding the phenomenon of plasma interactions with matter, coupled with the development of CAPP devices, have resulted in an interdisciplinary research topic of significant importance. This has led to the integration of various fields of science, including plasma physics, chemistry, biomedical sciences, and engineering. The reactive oxygen species and reactive nitrogen species generated from cold atmospheric plasma on interaction with biomolecules like proteins and peptides form various supramolecular structures. CAPP treatment of amino acids, which are the fundamental building blocks of proteins, holds potential in creating self-assembled supramolecular architectures. In this work, we demonstrate the process of self-assembly of aromatic amino acid tryptophan (Trp) enantiomers (l-tryptophan and d-tryptophan) into ordered supramolecular assemblies induced by the reactive species generated by a cold atmospheric pressure helium plasma jet. These enantiomers of tryptophan form organized structures as evidenced by FE-SEM. To assess the impact of CAPP treatment on the observed assemblies, we employed various analytical techniques such as zeta potential, dynamic light scattering and FTIR spectroscopy. Also, photoluminescence and time-resolved lifetime measurements revealed the transfiguration of individual Trp enantiomers. The LC-ESI-QTOF-MS analysis demonstrated that CAPP irradiation led to the incorporation of oxygenated ions into the pure Trp molecule. These studies of the self-assembly of Trp due to ROS and RNS interactions will help us to understand the assembly environment. This knowledge may be utilized to artificially design and synthesize highly ordered functional supramolecular structures using CAPP. Competing Interests: There are no conflicts to declare. (This journal is © The Royal Society of Chemistry.)
Databáze:	MEDLINE
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