| Autor: |
Negri EM; Departamento de Patologia, Faculdade de Medicina, Universidade de São Paulo, São Paulo, Brazil., Benchimol M; Laboratório de Ultraestrutura Celular Hertha Meyer, Instituto de Biofísica Carlos Chagas Filho, Centro Nacional de Biologia Estrutural e Bioimagens, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.; Universidade do Grande Rio (UNIGRANRIO), Rio de Janeiro, Brazil., Mauad T; Departamento de Patologia, Faculdade de Medicina, Universidade de São Paulo, São Paulo, Brazil., Duarte-Neto AN; Departamento de Patologia, Faculdade de Medicina, Universidade de São Paulo, São Paulo, Brazil., Gottardi M; Departamento de Patologia, Faculdade de Medicina, Universidade de São Paulo, São Paulo, Brazil., da Silva LFF; Departamento de Patologia, Faculdade de Medicina, Universidade de São Paulo, São Paulo, Brazil., Saldiva PHN; Departamento de Patologia, Faculdade de Medicina, Universidade de São Paulo, São Paulo, Brazil., Dolhnikoff M; Departamento de Patologia, Faculdade de Medicina, Universidade de São Paulo, São Paulo, Brazil., Souza W; Laboratório de Ultraestrutura Celular Hertha Meyer, Instituto de Biofísica Carlos Chagas Filho, Centro Nacional de Biologia Estrutural e Bioimagens, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.; Universidade do Grande Rio (UNIGRANRIO), Rio de Janeiro, Brazil., Garcia Caldini E; Departamento de Patologia, Faculdade de Medicina, Universidade de São Paulo, São Paulo, Brazil. |
| Abstrakt: |
Endothelial dysfunction is a key phenomenon in COVID-19, induced by direct viral endothelial infection and secondary inflammation, mainly affecting the microvascular circulation. However, few studies described the subcellular aspects of the lung microvasculature and the associated thrombotic phenomena, which are widely present in severe COVID-19 cases. To that end, in this transversal observational study we performed transmission and scanning electron microscopy in nine lung samples of patients who died due to COVID-19, obtained via minimally invasive autopsies in Sao Paulo, Brazil, in 2020. All patients died due to acute respiratory failure and had microvascular thrombosis at histology. Electron microscopy revealed areas of endothelial damage with basal lamina disruption and virus infection in endothelial cells. In the capillary lumens, the ultrastructure of the thrombi is depicted, with red blood cells stacking, dysmorphism and hemolysis, fibrin meshworks, and extracellular traps. Our description illustrates the complex pathophysiology of microvascular thrombosis at the cellular level, which leads to some of the peculiar characteristics of severe COVID-19. NEW & NOTEWORTHY In this study, electron microscopy was used to explain the pathophysiology of respiratory failure in severe COVID-19. Before the advent of vaccination, as the virus entered the respiratory system, it rapidly progressed to the alveolar capillary network and, before causing exudative alveolar edema, it caused mainly thrombosis of the pulmonary microcirculation with preserved lung compliance explaining "happy hypoxia." Timing of anticoagulation is of pivotal importance in this disease. |
