First identification and characterization of ovine gammaherpesvirus type 2 in horses and artiodactyla from an outbreak of malignant catarrhal fever in Mexico.

Autor: Madrigal-Valencia TL; Departamento de Microbiología e Inmunología, Facultad de Medicina Veterinaria y Zootecnia, UNAM, Mexico City, Mexico., Saavedra-Montañez M; Departamento de Microbiología e Inmunología, Facultad de Medicina Veterinaria y Zootecnia, UNAM, Mexico City, Mexico., Pérez-Torres A; Departamento de Biología Celular y Tisular, Facultad de Medicina, Universidad Nacional Autónoma de México (UNAM), Mexico City, Mexico., Hernández J; Laboratorio de Inmunología, Centro de Investigación en Alimentación y Desarrollo, A.C. (CIAD), Hermosillo, Sonora, Mexico., Segalés J; Unitat Mixta d'Investigació IRTA-UAB en Sanitat Animal, Centre de Recerca en Sanitat Animal (CReSA), Campus de la Universitat Autònoma de Barcelona (UAB), Bellaterra, Barcelona, Catalonia, España.; Department de Sanitat i Anatomia Animals, Facultat de Veterinària, Campus de la Universitat Autònoma de Barcelona (UAB), Bellaterra, Barcelona, Catalonia, España., Hernández YD; Centro de Enseñanza, investigación y Extensión en Producción Animal en Altiplano (CEIEPAA), Facultad de Medicina Veterinaria y Zootecnia (FMVZ), Universidad Nacional Autónoma de México (UNAM), Tequisquiapan, Queretaro, Mexico., Candanosa-Aranda IE; Centro de Enseñanza, investigación y Extensión en Producción Animal en Altiplano (CEIEPAA), Facultad de Medicina Veterinaria y Zootecnia (FMVZ), Universidad Nacional Autónoma de México (UNAM), Tequisquiapan, Queretaro, Mexico., Pérez-Guiot A; División de Ciencias de la Vida, Campus Irapuato-Salamanca, Universidad de Guanajuato, ExHda El Copal, Irapuato, Guanajuato, Mexico., Ramírez-Mendoza H; Departamento de Microbiología e Inmunología, Facultad de Medicina Veterinaria y Zootecnia, UNAM, Mexico City, Mexico.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2023 Sep 01; Vol. 18 (9), pp. e0290309. Date of Electronic Publication: 2023 Sep 01 (Print Publication: 2023).
DOI: 10.1371/journal.pone.0290309
Abstrakt: Ovine gammaherpesvirus 2 (OvHV-2), a member of the genus Macavirus, causes sheep-associated malignant catarrhal fever (SA-MCF), a fatal lymphoproliferative disease affecting a wide variety of ungulates in addition to horses. This study described an outbreak of SA-MCF in Mexico and the identification of the OvHV-2 virus in primary rabbit testis cultures through the generation of intranuclear inclusion bodies, syncytia, immunofluorescence (IF), immunocytochemistry (ICC), immunohistochemistry (IHC), endpoint polymerase chain reaction (PCR), and partial sequencing of the ORF75 gene. The animals involved in this outbreak showed mucogingival ulcers in the vestibule of the mouth and tongue, hypersalivation, corneal opacity, reduced food consumption, and weight loss of variable severity. These clinical signs and the histopathological findings suggested the diagnosis of SA-MCF. Buffy coat fractions from the anticoagulated blood samples of ill animals were collected and analyzed by PCR. Positive buffy coats were used to inoculate the primary cell cultures of rabbit testis to identify the virus. Small clusters of refractile cytomegalic cells, characteristic of viral cytopathic effects, were observed between 48 and 72 h post-infection. Furthermore, intranuclear acidophilic inclusion bodies (IBs) were identified in the inoculated primary culture cells, and the cytoplasm showed immunoreactivity with hyperimmune rabbit serum against OvHV-2. Moreover, in the liver histological sections from sick deer, immunoreactive juxtanuclear IBs were identified with the same rabbit hyperimmune serum. The obtained sequences were aligned with the OvHV-2 sequences reported in GenBank and revealed a nucleotide identity higher than 98%. Based on the evidence provided in this study, we conclude that the outbreak of SA-MCF in the municipality of Tequisquiapan in the state of Queretaro, Mexico, was caused by OvHV-2. This is the second study reporting that horses are susceptible to OvHV-2 infection and can develop SA-MCF. We identified for the first time in Mexico, the presence of OvHV-2 in buffy coats from horses and Artiodactyla.
Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. We would greatly appreciate your consideration for the manuscript entitled “Isolation and identification of Ovine gammaherpesvirus type 2 in an outbreak of Malignant Catarrhal Fever in Artiodactyla and horses in Mexico” for publication as a regular manuscript at PLOS ONE. This manuscript is product of the work of Tania Lucia Madrigal-Valencia, Manuel Saavedra-Montañez, Armando Pérez-Torres, Jesús Hernández, Joaquim Segalés, Yesmín Domínguez Hernández, Irma Eugenia Candanosa-Aranda, Alfredo Pérez-Guiot, and Humberto Ramírez-Mendoza. All of us have contributed to this work and we agree on its potential publication. The manuscript has been previously reviewed by Elsevier Language Editing Services (Order reference: ASLESTD0330355). We have agreed that Dr. Humberto Ramírez-Mendoza will act as corresponding author on our behalf regarding any subsequent processing of the paper.
(Copyright: © 2023 Madrigal-Valencia et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
Databáze: MEDLINE
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