Cell type-specific attenuation of brassinosteroid signaling precedes stomatal asymmetric cell division.

Autor:	Kim EJ; Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent 9052, Belgium.; Center for Plant Systems Biology, VIB, Ghent 9052, Belgium., Zhang C; Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent 9052, Belgium.; Center for Plant Systems Biology, VIB, Ghent 9052, Belgium., Guo B; Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent 9052, Belgium.; Center for Plant Systems Biology, VIB, Ghent 9052, Belgium.; College of Life Sciences, Wuhan University, Wuhan 430072, China., Eekhout T; Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent 9052, Belgium.; Center for Plant Systems Biology, VIB, Ghent 9052, Belgium.; VIB Single Cell Core, VIB, Ghent 9052, Belgium., Houbaert A; Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent 9052, Belgium.; Center for Plant Systems Biology, VIB, Ghent 9052, Belgium., Wendrich JR; Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent 9052, Belgium.; Center for Plant Systems Biology, VIB, Ghent 9052, Belgium., Vandamme N; VIB Single Cell Core, VIB, Ghent 9052, Belgium., Tiwari M; Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent 9052, Belgium.; Center for Plant Systems Biology, VIB, Ghent 9052, Belgium., Simon-Vezo C; Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent 9052, Belgium.; Center for Plant Systems Biology, VIB, Ghent 9052, Belgium., Vanhoutte I; Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent 9052, Belgium.; Center for Plant Systems Biology, VIB, Ghent 9052, Belgium., Saeys Y; Department of Applied Mathematics, Computer Science and Statistics, Ghent University, Ghent 9000, Belgium.; Data Mining and Modeling for Biomedicine, Center for Inflammation Research, VIB, Ghent 9052, Belgium., Wang K; Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent 9052, Belgium.; Center for Plant Systems Biology, VIB, Ghent 9052, Belgium.; College of Life Sciences, Wuhan University, Wuhan 430072, China., Zhu Y; College of Life Sciences, Wuhan University, Wuhan 430072, China., De Rybel B; Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent 9052, Belgium.; Center for Plant Systems Biology, VIB, Ghent 9052, Belgium., Russinova E; Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent 9052, Belgium.; Center for Plant Systems Biology, VIB, Ghent 9052, Belgium.
Jazyk:	angličtina
Zdroj:	Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2023 Sep 05; Vol. 120 (36), pp. e2303758120. Date of Electronic Publication: 2023 Aug 28.
DOI:	10.1073/pnas.2303758120
Abstrakt:	In Arabidopsis thaliana, brassinosteroid (BR) signaling and stomatal development are connected through the SHAGGY/GSK3-like kinase BR INSENSITIVE2 (BIN2). BIN2 is a key negative regulator of BR signaling but it plays a dual role in stomatal development. BIN2 promotes or restricts stomatal asymmetric cell division (ACD) depending on its subcellular localization, which is regulated by the stomatal lineage-specific scaffold protein POLAR. BRs inactivate BIN2, but how they govern stomatal development remains unclear. Mapping the single-cell transcriptome of stomatal lineages after triggering BR signaling with either exogenous BRs or the specific BIN2 inhibitor, bikinin, revealed that the two modes of BR signaling activation generate spatiotemporally distinct transcriptional responses. We established that BIN2 is always sensitive to the inhibitor but, when in a complex with POLAR and its closest homolog POLAR-LIKE1, it becomes protected from BR-mediated inactivation. Subsequently, BR signaling in ACD precursors is attenuated, while it remains active in epidermal cells devoid of scaffolds and undergoing differentiation. Our study demonstrates how scaffold proteins contribute to cellular signal specificity of hormonal responses in plants.
Databáze:	MEDLINE
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