Mechanical stimuli activate gene expression via a cell envelope stress sensing pathway.

Autor: Harper CE; Sibley School of Mechanical and Aerospace Engineering, Cornell University, Ithaca, NY, 14853, USA.; Meinig School of Biomedical Engineering, Cornell University, Ithaca, NY, 14853, USA., Zhang W; Department of Chemistry and Chemical Biology, Cornell University, Ithaca, NY, 14853, USA., Lee J; Sibley School of Mechanical and Aerospace Engineering, Cornell University, Ithaca, NY, 14853, USA., Shin JH; Weill Institute for Cell and Molecular Biology, Cornell University, Ithaca, NY, 14853, USA.; Department of Microbiology, Cornell University, Ithaca, NY, 14853, USA., Keller MR; Weill Institute for Cell and Molecular Biology, Cornell University, Ithaca, NY, 14853, USA., van Wijngaarden E; Sibley School of Mechanical and Aerospace Engineering, Cornell University, Ithaca, NY, 14853, USA., Chou E; Sibley School of Mechanical and Aerospace Engineering, Cornell University, Ithaca, NY, 14853, USA., Wang Z; Department of Chemistry and Chemical Biology, Cornell University, Ithaca, NY, 14853, USA., Dörr T; Weill Institute for Cell and Molecular Biology, Cornell University, Ithaca, NY, 14853, USA. tdoerr@cornell.edu.; Department of Microbiology, Cornell University, Ithaca, NY, 14853, USA. tdoerr@cornell.edu.; Cornell Institute of Host-Microbe Interactions and Disease, Cornell University, Ithaca, NY, 14853, USA. tdoerr@cornell.edu., Chen P; Department of Chemistry and Chemical Biology, Cornell University, Ithaca, NY, 14853, USA. pc252@cornell.edu., Hernandez CJ; Department of Bioengineering and Therapeutic Sciences and Orthopaedic Surgery, University of California, San Francisco, CA, 94143, USA. christopher.hernandez@ucsf.edu.; Chan Zuckerberg Biohub, San Francisco, CA, 94158, USA. christopher.hernandez@ucsf.edu.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2023 Aug 26; Vol. 13 (1), pp. 13979. Date of Electronic Publication: 2023 Aug 26.
DOI: 10.1038/s41598-023-40897-w
Abstrakt: Mechanosensitive mechanisms are often used to sense damage to tissue structure, stimulating matrix synthesis and repair. While this kind of mechanoregulatory process is well recognized in eukaryotic systems, it is not known whether such a process occurs in bacteria. In Vibrio cholerae, antibiotic-induced damage to the load-bearing cell wall promotes increased signaling by the two-component system VxrAB, which stimulates cell wall synthesis. Here we show that changes in mechanical stress within the cell envelope are sufficient to stimulate VxrAB signaling in the absence of antibiotics. We applied mechanical forces to individual bacteria using three distinct loading modalities: extrusion loading within a microfluidic device, direct compression and hydrostatic pressure. In all cases, VxrAB signaling, as indicated by a fluorescent protein reporter, was increased in cells submitted to greater magnitudes of mechanical loading, hence diverse forms of mechanical stimuli activate VxrAB signaling. Reduction in cell envelope stiffness following removal of the endopeptidase ShyA led to large increases in cell envelope deformation and substantially increased VxrAB response, further supporting the responsiveness of VxrAB. Our findings demonstrate a mechanosensitive gene regulatory system in bacteria and suggest that mechanical signals may contribute to the regulation of cell wall homeostasis.
(© 2023. Springer Nature Limited.)
Databáze: MEDLINE
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