Towards an understanding of the factors controlling bacterial diversity and activity in semi-passive Fe- and As-oxidizing bioreactors treating arsenic-rich acid mine drainage.

Autor:	Diaz-Vanegas C; HydroSciences Montpellier, University of Montpellier, CNRS, IRD, Montpellier, France.; French Geological Survey (BRGM), Water, Environment, Process and Analyses Division, Orléans, France., Héry M; HydroSciences Montpellier, University of Montpellier, CNRS, IRD, Montpellier, France., Desoeuvre A; HydroSciences Montpellier, University of Montpellier, CNRS, IRD, Montpellier, France., Bruneel O; HydroSciences Montpellier, University of Montpellier, CNRS, IRD, Montpellier, France., Joulian C; French Geological Survey (BRGM), Water, Environment, Process and Analyses Division, Orléans, France., Jacob J; French Geological Survey (BRGM), Water, Environment, Process and Analyses Division, Orléans, France., Battaglia-Brunet F; French Geological Survey (BRGM), Water, Environment, Process and Analyses Division, Orléans, France., Casiot C; HydroSciences Montpellier, University of Montpellier, CNRS, IRD, Montpellier, France.
Jazyk:	angličtina
Zdroj:	FEMS microbiology ecology [FEMS Microbiol Ecol] 2023 Aug 22; Vol. 99 (9).
DOI:	10.1093/femsec/fiad089
Abstrakt:	Semi-passive bioreactors based on iron and arsenic oxidation and coprecipitation are promising for the treatment of As-rich acid mine drainages. However, their performance in the field remains variable and unpredictable. Two bioreactors filled with distinct biomass carriers (plastic or a mix of wood and pozzolana) were monitored during 1 year. We characterized the dynamic of the bacterial communities in these bioreactors, and explored the influence of environmental and operational drivers on their diversity and activity. Bacterial diversity was analyzed by 16S rRNA gene metabarcoding. The aioA genes and transcripts were quantified by qPCR and RT-qPCR. Bacterial communities were dominated by several iron-oxidizing genera. Shifts in the communities were attributed to operational and physiochemical parameters including the nature of the biomass carrier, the water pH, temperature, arsenic, and iron concentrations. The bioreactor filled with wood and pozzolana showed a better resilience to disturbances, related to a higher bacterial alpha diversity. We evidenced for the first time aioA expression in a treatment system, associated with the presence of active Thiomonas spp. This confirmed the contribution of biological arsenite oxidation to arsenic removal. The resilience and the functional redundancy of the communities developed in the bioreactors conferred robustness and stability to the treatment systems. (© The Author(s) 2023. Published by Oxford University Press on behalf of FEMS.)
Databáze:	MEDLINE
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