| Autor: |
Touzout SN; Laboratory of Biodiversity, Biotechnologies and Climate Change (LR11/ES09), Faculty of Sciences of Tunis, University of Tunis El-Manar, Tunis 2092, Tunisia., Merghni A; Laboratory of Antimicrobial Resistance LR99ES09, Faculty of Medicine of Tunis, University of Tunis El Manar, Tunis 1007, Tunisia., Laouani A; Laboratory of Metabolic Biophysics and Applied Pharmacology, Faculty of Medicine of Sousse, University of Sousse, Sousse 4002, Tunisia.; USCR Analytical Platform UHPLC-MS &Research in Medicine and Biology, Faculty of Medicine of Sousse, University of Sousse, Sousse 4002, Tunisia., Boukhibar H; Laboratory of Biodiversity, Biotechnologies and Climate Change (LR11/ES09), Faculty of Sciences of Tunis, University of Tunis El-Manar, Tunis 2092, Tunisia., Alenazy R; Department of Medical Laboratory, College of Applied Medical Sciences-Shaqra, Shaqra University, Shaqra 11961, Saudi Arabia., Alobaid A; Department of Medical Laboratory, Aliman General Hospital-Riyadh, Ministry of Health, Riyadh 12684, Saudi Arabia., Alenazy M; Al-DAWAA Medical Company, Riyadh 14814, Saudi Arabia., Ben-Attia M; Environment Biomonitoring Laboratory (LR01/ES14), Faculty of Sciences of Bizerte, University of Carthage, Zarzouna 7021, Tunisia., Saguem K; Laboratory of Metabolic Biophysics and Applied Pharmacology, Faculty of Medicine of Sousse, University of Sousse, Sousse 4002, Tunisia.; USCR Analytical Platform UHPLC-MS &Research in Medicine and Biology, Faculty of Medicine of Sousse, University of Sousse, Sousse 4002, Tunisia., El-Bok S; Laboratory of Biodiversity, Biotechnologies and Climate Change (LR11/ES09), Faculty of Sciences of Tunis, University of Tunis El-Manar, Tunis 2092, Tunisia. |
| Abstrakt: |
Melia azedarach L., a Meliaceae family tree, is widely used in traditional folkloric medicine for its pharmaceutical properties. In the present study, we investigated the phytochemical composition of four methanolic leaf extracts of M. azedarach of various origins (Algeria and Tunisia) using high-performance liquid chromatography (HPLC). The antibacterial efficacy and mechanisms of action against Gram-positive and Gram-negative pathogenic microorganisms were then evaluated. Our findings revealed a presence of phenolic acids and flavonoids, such as gallic acid, chlorogenic acid, caffeic acid, hyperoside, isoquercetin, quercetin, and isorhamnetin both in Algerian and Tunisian localities, with an abundance of phenolic acids compared to flavonoids. Additionally, the studied extracts exhibit a broad spectrum of antibacterial activities, with MIC values ranging from 31.25 mg/mL to 125 mg/mL. Methanolic leaf extracts of M. azedarach from Algeria exhibited more potent biofilm eradication, with a percentage of inhibition reaching 72.17% against the S. aureus strain. Furthermore, inhibitory concentrations of tested substances, particularly the extract from the Relizane area, were capable of disrupting the membrane integrity of the treated bacteria as well as producing oxidative stress through ROS generation. Likewise, our results reveal that plant extract induces lipid peroxidation by raising MDA levels in comparison to untreated cells, particularly with the plant extract of Blida. M. azedarach extracts also reduced the synthesis of antioxidant enzymes (CAT and SOD). Our findings illustrate that M. azedarach remains a plant with significant antibacterial potential and distinct mechanisms of action that are closely related to the origins of this specimen. |
