| Autor: |
Moldovan M; Department of Physiology, Iuliu Hatieganu University of Medicine and Pharmacy, Clinicilor Street, No. 1, 400006 Cluj-Napoca, Romania., Păpurică AM; Department of Physiology, Iuliu Hatieganu University of Medicine and Pharmacy, Clinicilor Street, No. 1, 400006 Cluj-Napoca, Romania., Muntean M; Department of Cell and Molecular Biology, Iuliu Hatieganu University of Medicine and Pharmacy, Pasteur Street, No. 6, 400349 Cluj-Napoca, Romania., Bungărdean RM; Department of Pathology, Iuliu Hatieganu University of Medicine and Pharmacy, Clinicilor Street, No. 3-5, 400340 Cluj-Napoca, Romania., Gheban D; Department of Pathology, Iuliu Hatieganu University of Medicine and Pharmacy, Clinicilor Street, No. 3-5, 400340 Cluj-Napoca, Romania.; Department of Pathology, Emergency Clinical Hospital for Children, Motilor Street, No. 41T-42T, 400370 Cluj-Napoca, Romania., Moldovan B; Faculty of Chemistry and Chemical Engineering, Babes-Bolyai University, Arany Janos Street, No. 11, 400028 Cluj-Napoca, Romania., Katona G; Faculty of Chemistry and Chemical Engineering, Babes-Bolyai University, Arany Janos Street, No. 11, 400028 Cluj-Napoca, Romania., David L; Faculty of Chemistry and Chemical Engineering, Babes-Bolyai University, Arany Janos Street, No. 11, 400028 Cluj-Napoca, Romania., Filip GA; Department of Physiology, Iuliu Hatieganu University of Medicine and Pharmacy, Clinicilor Street, No. 1, 400006 Cluj-Napoca, Romania. |
| Abstrakt: |
Diabetic retinopathy (DR) and cataracts (CA) have an early onset in diabetes mellitus (DM) due to the redox imbalance and inflammation triggered by hyperglycaemia. Plant-based therapies are characterised by low tissue bioavailability. The study aimed to investigate the effect of gold nanoparticles phytoreduced with Rutin (AuNPsR), as a possible solution. Insulin, Rutin, and AuNPsR were administered to an early, six-week rat model of DR and CA. Oxidative stress (MDA, CAT, SOD) was assessed in serum and eye homogenates, and inflammatory cytokines (IL-1 beta, IL-6, TNF alpha) were quantified in ocular tissues. Eye fundus of retinal arterioles, transmission electron microscopy (TEM) of lenses, and histopathology of retinas were also performed. DM was linked to constricted retinal arterioles, reduced endogen antioxidants, and eye inflammation. Histologically, retinal wall thickness decreased. TEM showed increased lens opacity and fibre disorganisation. Rutin improved retinal arteriolar diameter, while reducing oxidative stress and inflammation. Retinas were moderately oedematous. Lens structure was preserved on TEM. Insulin restored retinal arteriolar diameter, while increasing MDA, and amplifying TEM lens opacity. The best outcomes were obtained for AuNPsR, as it improved fundus appearance of retinal arterioles, decreased MDA and increased antioxidant capacity. Retinal edema and disorganisation in lens fibres were still present. |
