Bacterial Lipopolysaccharides Exacerbate Neurogenic Heterotopic Ossification Development.

Autor: Salga M; Mater Research Institute-The University of Queensland, Translational Research Institute, Woolloongabba, Australia.; University of Versailles Saint Quentin en Yvelines, END:ICAP U1179 INSERM, UFR Simone Veil-Santé, Montigny le Bretonneux, France.; UPOH (Unité Péri Opératoire du Handicap), Physical and Rehabilitation Medicine Department, Raymond-Poincaré Hospital, Assistance Publique-Hôpitaux de Paris (AP-HP), Garches, France., Samuel SG; Mater Research Institute-The University of Queensland, Translational Research Institute, Woolloongabba, Australia.; Department of Oral Pathology and Microbiology, Saveetha Dental College and Hospitals, Chennai, India., Tseng HW; Mater Research Institute-The University of Queensland, Translational Research Institute, Woolloongabba, Australia., Gatin L; University of Versailles Saint Quentin en Yvelines, END:ICAP U1179 INSERM, UFR Simone Veil-Santé, Montigny le Bretonneux, France.; UPOH (Unité Péri Opératoire du Handicap), Physical and Rehabilitation Medicine Department, Raymond-Poincaré Hospital, Assistance Publique-Hôpitaux de Paris (AP-HP), Garches, France.; Department of Orthopedic Surgery, Raymond Poincaré Hospital, AP-HP, Garches, France., Girard D; Institut de Recherche Biomédicale des Armées (IRBA), INSERM UMR-MD 1197, Clamart, France., Rival B; Institut de Recherche Biomédicale des Armées (IRBA), INSERM UMR-MD 1197, Clamart, France., Barbier V; Mater Research Institute-The University of Queensland, Translational Research Institute, Woolloongabba, Australia., Bisht K; Mater Research Institute-The University of Queensland, Translational Research Institute, Woolloongabba, Australia., Shatunova S; Mater Research Institute-The University of Queensland, Translational Research Institute, Woolloongabba, Australia., Debaud C; University of Versailles Saint Quentin en Yvelines, END:ICAP U1179 INSERM, UFR Simone Veil-Santé, Montigny le Bretonneux, France., Winkler IG; Mater Research Institute-The University of Queensland, Translational Research Institute, Woolloongabba, Australia., Paquereau J; UPOH (Unité Péri Opératoire du Handicap), Physical and Rehabilitation Medicine Department, Raymond-Poincaré Hospital, Assistance Publique-Hôpitaux de Paris (AP-HP), Garches, France., Dinh A; Department of Infectious Diseases, Raymond Poincaré Hospital, AP-HP, Garches, France., Genêt G; University of Versailles Saint Quentin en Yvelines, END:ICAP U1179 INSERM, UFR Simone Veil-Santé, Montigny le Bretonneux, France., Kerever S; Department of Anesthesiology and Critical Care, Lariboisière University Hospital, AP-HP, Paris, France., Abback PS; Department of Anesthesiology and Critical Care, Beaujon Hospital, DMU Parabol, AP-HP, Clichy, France., Banzet S; Institut de Recherche Biomédicale des Armées (IRBA), INSERM UMR-MD 1197, Clamart, France., Genêt F; University of Versailles Saint Quentin en Yvelines, END:ICAP U1179 INSERM, UFR Simone Veil-Santé, Montigny le Bretonneux, France.; UPOH (Unité Péri Opératoire du Handicap), Physical and Rehabilitation Medicine Department, Raymond-Poincaré Hospital, Assistance Publique-Hôpitaux de Paris (AP-HP), Garches, France., Lévesque JP; Mater Research Institute-The University of Queensland, Translational Research Institute, Woolloongabba, Australia., Alexander KA; Mater Research Institute-The University of Queensland, Translational Research Institute, Woolloongabba, Australia.
Jazyk: angličtina
Zdroj: Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research [J Bone Miner Res] 2023 Nov; Vol. 38 (11), pp. 1700-1717. Date of Electronic Publication: 2023 Sep 23.
DOI: 10.1002/jbmr.4905
Abstrakt: Neurogenic heterotopic ossifications (NHO) are heterotopic bones that develop in periarticular muscles after severe central nervous system (CNS) injuries. Several retrospective studies have shown that NHO prevalence is higher in patients who suffer concomitant infections. However, it is unclear whether these infections directly contribute to NHO development or reflect the immunodepression observed in patients with CNS injury. Using our mouse model of NHO induced by spinal cord injury (SCI) between vertebrae T 11 to T 13 , we demonstrate that lipopolysaccharides (LPS) from gram-negative bacteria exacerbate NHO development in a toll-like receptor-4 (TLR4)-dependent manner, signaling through the TIR-domain-containing adapter-inducing interferon-β (TRIF/TICAM1) adaptor rather than the myeloid differentiation primary response-88 (MYD88) adaptor. We find that T 11 to T 13 SCI did not significantly alter intestinal integrity nor cause intestinal bacteria translocation or endotoxemia, suggesting that NHO development is not driven by endotoxins from the gut in this model of SCI-induced NHO. Relevant to the human pathology, LPS increased expression of osteoblast markers in cultures of human fibro-adipogenic progenitors isolated from muscles surrounding NHO biopsies. In a case-control retrospective study in patients with traumatic brain injuries, infections with gram-negative Pseudomonas species were significantly associated with NHO development. Together these data suggest a functional association between gram-negative bacterial infections and NHO development and highlights infection management as a key consideration to avoid NHO development in patients. © 2023 The Authors. Journal of Bone and Mineral Research published by Wiley Periodicals LLC on behalf of American Society for Bone and Mineral Research (ASBMR).
(© 2023 The Authors. Journal of Bone and Mineral Research published by Wiley Periodicals LLC on behalf of American Society for Bone and Mineral Research (ASBMR).)
Databáze: MEDLINE
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