A new cyclodipeptide from Tetragonisca angustula honey active against Neospora caninum and in silico study.

Autor: de Oliveira Lima ÂC; Graduate Program in Biotechnology, State University of Feira de Santana - UEFS, Feira de Santana, BA, Brazil.; VeterinaryBiochemistry and Immunology Laboratory, Center for Agricultural, Environmental and Biological Sciences, Federal University of Reconcavo da Bahia (UFRB), Cruz das Almas, BA, Brazil., Nolasco M; Graduate Program in Biotechnology, State University of Feira de Santana - UEFS, Feira de Santana, BA, Brazil., Freitas LDS; VeterinaryBiochemistry and Immunology Laboratory, Center for Agricultural, Environmental and Biological Sciences, Federal University of Reconcavo da Bahia (UFRB), Cruz das Almas, BA, Brazil., Pinheiro AM; VeterinaryBiochemistry and Immunology Laboratory, Center for Agricultural, Environmental and Biological Sciences, Federal University of Reconcavo da Bahia (UFRB), Cruz das Almas, BA, Brazil., de Carvalho CAL; Center for Agricultural, Environmental and Biological Sciences, Federal University of Reconcavo da Bahia (UFRB), Cruz das Almas, BA, Brazil., de Freitas HF; Department of Health, State University of Feira de Santana, Feira de Santana, BA, Brazil.; Laboratory of Bioinformatics and Molecular Modeling (LaBiMM,), Pharmacy College, Federal University of Bahia (UFBA), Salvador, Bahia, Brazil., Pita SSDR; Laboratory of Bioinformatics and Molecular Modeling (LaBiMM,), Pharmacy College, Federal University of Bahia (UFBA), Salvador, Bahia, Brazil., Vieira IJC; Department of Natural Products Chemistry -LCQUI-CCT, University State of North Fluminense Darcy Ribeiro, Campos dos Goytacazes, RJ, Brazil., Braz Filho R; Department of Natural Products Chemistry -LCQUI-CCT, University State of North Fluminense Darcy Ribeiro, Campos dos Goytacazes, RJ, Brazil.; Chemistry Department -DEQUIM-ICE, Federal Rural University of Rio de Janeiro, Seropédica, RJ, Brazil., Branco A; Department of Health, State University of Feira de Santana, Feira de Santana, BA, Brazil.
Jazyk: angličtina
Zdroj: Natural product research [Nat Prod Res] 2024 Aug; Vol. 38 (16), pp. 2909-2914. Date of Electronic Publication: 2023 Aug 10.
DOI: 10.1080/14786419.2023.2245538
Abstrakt: A new cyclic natural compound formed by succinic acid and two alanine amino acid units was isolated from the Tetragonisca angustula honey extract. The chemical structure of 1 was established based on spectroscopic data analysis, including one- ( 1 H and 13 C NMR) and two-dimensional NMR techniques ( 1 H- 1 H-COSY, HSQC and HMBC). A primary culture model previously infected with Neospora caninum was used to evaluate 1 for two time intervals (24 and 72 h), showing a reduction (40-56%) of the number of tachyzoites in the first 24 h and until 72 h, a dose-dependent reduction in parasite proliferation (25-50%). Glial cells treated with 1 did not demonstrate toxicity at concentrations up to 25 ug/mL. Treated and infected cultures showed an increase in NO when compared to control cells in 24 h and 72 h. In silico studies suggest that the new compound may affect DNA synthesis and impair -protein production.
Databáze: MEDLINE
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