Fibrin deposition on bovine pericardium tissue used for bioprosthetic heart valve drives its calcification.
| Autor: | Poitier B; Université de Paris Cité, Innovative Therapies in Haemostasis, INSERM UMR-S1140, Paris, France.; Cardiovascular Surgery Department, AP-HP, Georges Pompidou European Hospital, Paris, France.; Biosurgical Research Lab (Carpentier Foundation, Université de Paris Cité and AP-HP), Paris, France., Rancic J; Université de Paris Cité, Innovative Therapies in Haemostasis, INSERM UMR-S1140, Paris, France., Richez U; Université de Paris Cité, Innovative Therapies in Haemostasis, INSERM UMR-S1140, Paris, France., Piquet J; Biosurgical Research Lab (Carpentier Foundation, Université de Paris Cité and AP-HP), Paris, France., El Batti S; Université de Paris Cité, Innovative Therapies in Haemostasis, INSERM UMR-S1140, Paris, France.; Cardiovascular Surgery Department, AP-HP, Georges Pompidou European Hospital, Paris, France.; Biosurgical Research Lab (Carpentier Foundation, Université de Paris Cité and AP-HP), Paris, France., Smadja DM; Université de Paris Cité, Innovative Therapies in Haemostasis, INSERM UMR-S1140, Paris, France.; Biosurgical Research Lab (Carpentier Foundation, Université de Paris Cité and AP-HP), Paris, France.; Hematology Department, AP-HP, Georges Pompidou European Hospital, Paris, France. |
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| Jazyk: | angličtina |
| Zdroj: | Frontiers in cardiovascular medicine [Front Cardiovasc Med] 2023 Jul 31; Vol. 10, pp. 1198020. Date of Electronic Publication: 2023 Jul 31 (Print Publication: 2023). |
| DOI: | 10.3389/fcvm.2023.1198020 |
| Abstrakt: | Background: Bioprosthetic heart valves (BHVs) are less thrombogenic than mechanical prostheses; however, BHV thrombosis has been proposed as a risk factor for premature BHV degeneration. Objectives: We aimed to explore whether fibrin deposition on bovine pericardium tissue could lead to calcification. Method: Fibrin clot was obtained by blending three reagents, namely, CRYOcheck™ Pooled Normal Plasma (4/6), tissue factor + phospholipids (Thrombinoscope BV), and 100 mM calcium (1/6), and deposited on pericardium discs. Non-treated and fibrin-treated bovine pericardium discs were inserted into the subcutaneous tissue of 12-day-old Wistar rats and sequentially explanted on days 5, 10, and 15. Calcium content was measured with acetylene flame atomic absorption spectrophotometry. Histological analysis was performed using hematoxylin-eosin staining, Von Kossa staining, and immunohistochemistry. Results: Calcification levels were significantly higher in fibrin-treated bovine pericardium discs compared to those in non-treated bovine pericardium discs (27.45 ± 23.05 µg/mg vs. 6.34 ± 6.03 µg/mg on day 5, 64.34 ± 27.12 µg/mg vs. 34.21 ± 19.11 µg/mg on day 10, and 64.34 ± 27.12 µg/mg vs. 35.65 ± 17.84 µg/mg on day 15; p < 0.001). Von Kossa staining confirmed this finding. In hematoxylin-eosin staining, the bovine pericardium discs were more extensively and deeply colonized by inflammatory-like cells, particularly T lymphocytes (CD3 + cells), when pretreated with fibrin. Conclusion: Fibrin deposition on bovine pericardium tissue treated with glutaraldehyde, used for BHV, led to increased calcification in a rat model. BHV thrombosis could be one of the triggers for calcification and BHV deterioration. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (© 2023 Poitier, Rancic, Richez, Piquet, El Batti and Smadja.) |
| Databáze: | MEDLINE |
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