Nuclear factor erythrogen-2 associated factor 2 (Nrf2) signaling is an essential molecular pathway for the anti-aging effect of whey protein in the prefrontal cortex of aging rat model (Histological and Biochemical Study).

Autor: Sarhan NR; Medical Histology and Cell Biology Department, Faculty of Medicine, Mansoura University, Egypt; Medical Histology and Cell Biology Department, Faculty of Medicine, Horus University - Egypt. Electronic address: nahlaredasarhan@mans.edu.eg., El Nashar EM; Department of Anatomy, college of Medicine, King Khalid University, Abha 61421, Saudi Arabia., Hamza E; Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Mansoura University, Egypt; Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Horus University - Egypt., El-Beah SM; Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Mansoura University, Egypt; Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Badr University in Cairo, Egypt., Alghamdi MA; Department of Anatomy, college of Medicine, King Khalid University, Abha 61421, Saudi Arabia; Genomics and Personalized Medicine Unit, college of Medicine, King Khalid University, Abha 61421, Saudi Arabia., Al-Khater KM; Department of Anatomy, College of Medicine, Imam Abdulrahman Bin Faisal University, P.O. Box, 2114, Dammam 31451, Saudi Arabia., Aldahhan RA; Department of Anatomy, College of Medicine, Imam Abdulrahman Bin Faisal University, P.O. Box, 2114, Dammam 31451, Saudi Arabia., Abul-Ela ES; Medical Histology and Cell Biology Department, Faculty of Medicine, Mansoura University, Egypt.
Jazyk: angličtina
Zdroj: Tissue & cell [Tissue Cell] 2023 Oct; Vol. 84, pp. 102192. Date of Electronic Publication: 2023 Aug 09.
DOI: 10.1016/j.tice.2023.102192
Abstrakt: Aging is a highly complicated natural process. Brain aging is associated with remarkable neurodegenerative changes and oxidative damage. Whey protein (WP) has been mentioned to have an antioxidant property. Nuclear factor erythrogen-2 associated factor 2 (Nrf2) signaling pathway is an antioxidant defense system. Nrf2 activity declines with age so, its activation could be a promising therapeutic strategy for aging. This study aimed to explore the anti-aging role of WP against D-galactose (D-gal) induced age-related degenerative changes and oxidative damage in the prefrontal cortex (PFC) and investigate its underlying mechanisms. Forty adult male rats were divided into 4 groups; control, WP group received WP (28.77 mg/kg/day) by gastric tube on the 4 th experimental week; D-gal (model group) received D-gal (300 mg/kg/day) intraperitoneally for 8 weeks and D-gal +WP group received WP on the 4 th week of D-gal treatment. Specimens from PFC were obtained for biochemical, histological, immunohistochemical and western blot analysis. WP treatment in D-gal +WP group reduced lipid peroxidation, enhanced antioxidant enzyme activities, decreased advanced glycation end products level and improved the histological and ultrastructural alterations. Moreover, the number of neurons expressed the senescence marker; p21 and percentage area of the astrocytic marker; glial fibrillary acidic protein were significantly reduced. WP also enhanced Nrf2 pathway and its downstream targets; heme oxygenase-1 and NADPH quinone oxidoreductase 1. In conclusion WP alleviates the D-gal-induced PFC aging through activating Nrf2 pathway, reducing cell senescence and gliosis. So, it may be a potential therapeutic target to retard the aging process.
Competing Interests: Declaration of Competing Interest The authors declare no competing interests.
(Copyright © 2023 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: