| Autor: |
Beydoun S; Molecular and Integrative Physiology Department, University of Michigan, Ann Arbor., Kitto ES; Molecular and Integrative Physiology Department, University of Michigan, Ann Arbor., Wang E; Molecular and Integrative Physiology Department, University of Michigan, Ann Arbor., Huang S; Molecular and Integrative Physiology Department, University of Michigan, Ann Arbor., Leiser SF; Molecular and Integrative Physiology Department, University of Michigan, Ann Arbor; Department of Internal Medicine, University of Michigan, Ann Arbor; leiser@umich.edu. |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of visualized experiments : JoVE [J Vis Exp] 2023 Jul 28 (197). Date of Electronic Publication: 2023 Jul 28. |
| DOI: |
10.3791/65775 |
| Abstrakt: |
Caenorhabditis elegans is a common model organism for research in genetics, development, aging, metabolism, and behavior. Because C. elegans consume a diet of live bacteria, the metabolic activity of their food source can confound experiments looking for the direct effects of various interventions on the worm. To avoid the confounding effects of bacterial metabolism, C. elegans researchers have used multiple methods to metabolically inactivate bacteria, including ultraviolet (UV)-irradiation, heat-killing, and antibiotics. UV treatment is relatively low-throughput and cannot be used in liquid culture because each plate must be examined for successful bacterial killing. A second treatment method, heat-killing, negatively affects the texture and nutritional quality of the bacteria, leading to the developmental arrest of C. elegans. Finally, antibiotic treatment can directly alter C. elegans physiology in addition to preventing bacterial growth. This manuscript describes an alternative method to metabolically inactivate bacteria using paraformaldehyde (PFA). PFA treatment cross-links proteins within bacterial cells to prevent metabolic activity while preserving cellular structure and nutritional content. This method is high-throughput and can be used in liquid culture or solid plates, as testing one plate of PFA-treated bacteria for growth validates the whole batch. Metabolic inactivation through PFA treatment can be used to eliminate the confounding effects of bacterial metabolism on studies of drug or metabolite supplementation, stress resistance, metabolomics, and behavior in C. elegans. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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