Assessing the Biocompatibility and Regeneration of Electrospun-Nanofiber Composite Tracheal Grafts.

Autor: Kreber L; College of Medicine, The Ohio State University, Columbus, Ohio, U.S.A., Liu L; Center for Regenerative Medicine, Abigail Wexner Research Institute, Nationwide Children's Hospital, Columbus, Ohio, U.S.A., Dharmadhikari S; College of Medicine, The Ohio State University, Columbus, Ohio, U.S.A.; Center for Regenerative Medicine, Abigail Wexner Research Institute, Nationwide Children's Hospital, Columbus, Ohio, U.S.A., Tan ZH; College of Medicine, The Ohio State University, Columbus, Ohio, U.S.A.; Department of Otolaryngology - Head and Neck Surgery, Nationwide Children's Hospital, Columbus, Ohio, U.S.A., Chan C; College of Medicine, The Ohio State University, Columbus, Ohio, U.S.A., Huddle J; Nanofiber Solutions, Inc., Dublin, Ohio, U.S.A., Hussein Z; College of Medicine, The Ohio State University, Columbus, Ohio, U.S.A., Shontz K; Center for Regenerative Medicine, Abigail Wexner Research Institute, Nationwide Children's Hospital, Columbus, Ohio, U.S.A., Breuer CK; Center for Regenerative Medicine, Abigail Wexner Research Institute, Nationwide Children's Hospital, Columbus, Ohio, U.S.A.; Department of Pediatric Surgery, Nationwide Children's Hospital, Columbus, Ohio, U.S.A., Johnson J; Nanofiber Solutions, Inc., Dublin, Ohio, U.S.A., Chiang T; College of Medicine, The Ohio State University, Columbus, Ohio, U.S.A.; Center for Regenerative Medicine, Abigail Wexner Research Institute, Nationwide Children's Hospital, Columbus, Ohio, U.S.A.; Department of Otolaryngology - Head and Neck Surgery, Nationwide Children's Hospital, Columbus, Ohio, U.S.A.
Jazyk: angličtina
Zdroj: The Laryngoscope [Laryngoscope] 2024 Mar; Vol. 134 (3), pp. 1155-1162. Date of Electronic Publication: 2023 Aug 14.
DOI: 10.1002/lary.30955
Abstrakt: Objective: Composite tracheal grafts (CTG) combining decellularized scaffolds with external biomaterial support have been shown to support host-derived neotissue formation. In this study, we examine the biocompatibility, graft epithelialization, vascularization, and patency of three prototype CTG using a mouse microsurgical model.
Study Design: Tracheal replacement, regenerative medicine, biocompatible airway splints, animal model.
Method: CTG electrospun splints made by combining partially decellularized tracheal grafts (PDTG) with polyglycolic acid (PGA), poly(lactide-co-ε-caprolactone) (PLCL), or PLCL/PGA were orthotopically implanted in mice (N = 10/group). Tracheas were explanted two weeks post-implantation. Micro-Computed Tomography was conducted to assess for graft patency, and histological analysis was used to assess for epithelialization and neovascularization.
Result: Most animals (greater than 80%) survived until the planned endpoint and did not exhibit respiratory symptoms. MicroCT confirmed the preservation of graft patency. Grossly, the PDTG component of CTG remained intact. Examining the electrospun component of CTG, PGA degraded significantly, while PLCL+PDTG and PLCL/PGA + PDTG maintained their structure. Microvasculature was observed across the surface of CTG and infiltrating the pores. There were no signs of excessive cellular infiltration or encapsulation. Graft microvasculature and epithelium appear similar in all groups, suggesting that CTG did not hinder endothelialization and epithelialization.
Conclusion: We found that all electrospun nanofiber CTGs are biocompatible and did not affect graft patency, endothelialization and epithelialization. Future directions will explore methods to accelerate graft regeneration of CTG.
Level of Evidence: N/A Laryngoscope, 134:1155-1162, 2024.
(© 2023 The American Laryngological, Rhinological and Otological Society, Inc.)
Databáze: MEDLINE
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