Detection of Multiplex NASBA RNA Products Using Colorimetric Split G Quadruplex Probes.

Autor:	Rubel MS; Laboratory of DNA-Nanosensor Diagnostics, ITMO University, Saint Petersburg, Russia. rubel@scamt-itmo.ru., Shkodenko LA; Laboratory of DNA-Nanosensor Diagnostics, ITMO University, Saint Petersburg, Russia., Gorbenko DA; Laboratory of DNA-Nanosensor Diagnostics, ITMO University, Saint Petersburg, Russia., Solyanikova VV; Laboratory of DNA-Nanosensor Diagnostics, ITMO University, Saint Petersburg, Russia., Maltzeva YI; Laboratory of DNA-Nanosensor Diagnostics, ITMO University, Saint Petersburg, Russia., Rubel AA; Laboratory of Amyloid Biology, Saint-Petersburg State University, Saint Petersburg, Russia., Koshel EI; Laboratory of DNA-Nanosensor Diagnostics, ITMO University, Saint Petersburg, Russia., Kolpashchikov DM; Department of Chemistry, University of Central Florida, Orlando, FL, USA.; Burnett School of Biomedical Sciences, University of Central Florida, Orlando, FL, USA.; Center for Forensic Science, University of Central Florida, Orlando, FL, USA.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2023; Vol. 2709, pp. 287-298.
DOI:	10.1007/978-1-0716-3417-2_20
Abstrakt:	Structural RNA is a challenging target for recognition by hybridization probes. This chapter addresses the recognition problem of RNA amplicons in samples obtained by multiplex nucleic acid sequence-based amplification (NASBA). The method describes the design of G-quadruplex binary (split) DNA peroxidase sensors that produces colorimetric signal upon recognition of NASBA amplicons. (© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze:	MEDLINE
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