| Autor: |
Ghodke J; College of Health, Science and Society, University of the West of England, Coldharbour Ln, Bristol BS16 1QY, UK., Ekonomou SI; College of Health, Science and Society, University of the West of England, Coldharbour Ln, Bristol BS16 1QY, UK., Weaver E; School of Pharmacy, Queen's University Belfast, 97 Lisburn Road, Belfast BT9 7BL, UK., Lamprou D; School of Pharmacy, Queen's University Belfast, 97 Lisburn Road, Belfast BT9 7BL, UK., Doran O; College of Health, Science and Society, University of the West of England, Coldharbour Ln, Bristol BS16 1QY, UK., Stratakos AC; College of Health, Science and Society, University of the West of England, Coldharbour Ln, Bristol BS16 1QY, UK. |
| Abstrakt: |
In this study, liposomes enclosing eugenol were prepared using microfluidics. Two lipids-1,2-dimyristoyl- sn -glycero-3-phosphocholine, 18:0 (DSPC) and 2-dimyristoyl- sn -glycero-3-phosphocholine, 14:0 (DMPC)-and microfluidic chips with serpentine and Y-shaped micromixing designs were used for the liposomal formulation. Minimum bactericidal concentration (MBC) values indicated that eugenol was more effective against Gram-negative than Gram-positive bacteria. Four different flow-rate ratios (FRR 2:1, 3:1, 4:1, 5:1) were explored. All liposomes' encapsulation efficiency (EE) was determined: 94.34% for DSPC 3:1 and 78.63% for DMPC 5:1. The highest eugenol release of 99.86% was observed at pH 4, DMPC 3:1 (Y-shaped chip). Liposomes were physically stable at 4, 20 and 37 °C for 60 days as determined by their size, polydispersity index (PDI) and zeta potential (ZP). The most stable liposomes were observed at FRR 5:1 for DSPC. EE, stability, and eugenol release studies proved that the liposomal formulations produced can be used as delivery vehicles to increase food safety. |
