Chitosan thread in the healing of the cecal wall of rabbits (Oryctolagus cuniculus) submitted to cecorrhaphy.

Autor: Ávila Filho SH; Department of Veterinary Surgery, Federal University Goiás and Goiano Federal Institute, Goiás, Brazil., Moura VMBD; Department of Veterinary Pathology, Federal University Goiás, Goiás, Brazil., Queiroz PJB; Department of Veterinary Surgery, Federal University Goiás, Goiás, Brazil., Faleiro MBR; Department of Veterinary Pathology, Federal University Goiás, Goiás, Brazil., Lima BSG; Department of Veterinary Pathology, Federal University Goiás, Goiás, Brazil., Freitas SLR; Department of Veterinary Surgery, Goiano Federal Institute, Goiás, Brazil., Santos AS; Department of Veterinary Pathology, Goiano Federal Institute, Goiás, Brazil., Silva LAF; Department of Veterinary Surgery, Federal University Goiás, Goiás, Brazil.
Jazyk: angličtina
Zdroj: Journal of biomedical materials research. Part B, Applied biomaterials [J Biomed Mater Res B Appl Biomater] 2024 Jan; Vol. 112 (1), pp. e35314. Date of Electronic Publication: 2023 Aug 11.
DOI: 10.1002/jbm.b.35314
Abstrakt: The development and evaluation of synthesis materials are crucial to reducing the morbidity and magnitude of post-enterorrhaphy surgical complications. Despite the possibility of production, chitosan thread has not yet been used in enterorrhaphy, and its effects on intestinal healing have not been evaluated. Therefore, this study aimed to evaluate the effects of chitosan thread on the intestinal wall repair of rabbits submitted to cecorrhaphy. For this, 42 rabbits were allocated into two groups with 21 animals. One group was submitted to cecorrhaphy with chitosan suture thread (CG) and the other with poliglecaprone suture thread (PG). The occurrence of postoperative complications, the intensity of edema, cellular response, formation of granulation tissue, as well as the deposition and maturation of collagen fibers, and the intensity of vascular endothelial growth factor (VEGF-α) expression, were evaluated during the intestinal wall repair process. The evaluations occurred on the 5th, 15th, and 25th postoperative (PO) days. The animals did not develop peritonitis, but adherence was observed in six animals from CG and seven from PG, with no difference between groups. The polymorphonuclear infiltrate showed higher intensity and higher amount of type III collagen fibers in CG on the 15th PO day. In contrast, a lower amount of type I collagen fibers was observed in CG samples on the 25th PO day. Therefore, the chitosan thread used for cecorrhaphy in rabbits results in minimal postoperative complications, presents biocompatibility, and bioactively assists the tissue repair process of the cecal wall, inducing minimal tissue reaction, stimulating the deposition of type III collagen fibers in the proliferative phase, with sustained VEGF-α expression, but with reduced deposition of type I fibers, indicating a delay in collagen maturation.
(© 2023 Wiley Periodicals LLC.)
Databáze: MEDLINE
Externí odkaz: