| Autor: |
Rossi I; Department of Pharmaceutical and Pharmacological Sciences (DSF), University of Padova, Padova, Italy., Barollo S; Endocrinology Unit, Department of Medicine (DIMED), University of Padova, Padova, Italy., Bertazza L; Endocrinology Unit, Department of Medicine (DIMED), University of Padova, Padova, Italy., Mondin A; Endocrinology Unit, Department of Medicine (DIMED), University of Padova, Padova, Italy., Pedron MC; Endocrinology Unit, Department of Medicine (DIMED), University of Padova, Padova, Italy., Marodin G; Department of Pharmaceutical and Pharmacological Sciences (DSF), University of Padova, Padova, Italy., Lupo MG; Endocrinology Unit, Department of Medicine (DIMED), University of Padova, Padova, Italy., Palumbo M; Department of Food and Drug, University of Parma, Parma, Italy., Adorni MP; Department of Medicine and Surgery, University of Parma, Parma, Italy., Mian C; Endocrinology Unit, Department of Medicine (DIMED), University of Padova, Padova, Italy., Pezzani R; Phytotherapy Lab, Department of Medicine (DIMED), University of Padova, Padova, Italy.; AIROB, Associazione Italiana per la Ricerca Oncologica di Base, Padova, Italy., Ferri N; Endocrinology Unit, Department of Medicine (DIMED), University of Padova, Padova, Italy.; Veneto Institute of Molecular Medicine (VIMM), Padua, Italy. |
| Abstrakt: |
Handroanthus impetiginosus (Mart. ex DC.) Mattos is a plant from Central-South America that possesses different pharmacological activities. Plant extract was tested in THP-1 cell model, a human cell line used to study monocyte/macrophage functions. First, the plant effects on cell viability were evaluated, demonstrating no harmful consequences even at the higher concentrations (200 µg/ml). Thus, anti-inflammatory activity was investigated in gene and protein expression by RT-qPCR and ELISA methods, resulting in a reduction of pro-inflammatory cytokines after Handroanthus impetiginosus treatment. Similarly, NF-kB nuclear translocation was decreased according to confocal images and ImageStream X -analysis. Subsequently, in macrophage differentiated THP-1, CD36 mRNA and protein expression was inhibited in a concentration-dependent manner together with cell morphology changes during treatment. In addition, modified LDL-derived cholesterol uptake by THP-1 cells was reduced after plant extract incubation. Handroanthus impetiginosus showed anti-inflammatory and immunomodulating properties that may pave the way for future characterization in higher models. |
