| Autor: |
Tannous LA; School of Medicine, Pontifícia Universidade Católica do Paraná, Curitiba 80215-901, Paraná, Brazil., Westphal GA; State Transplant Central, Florianópolis 88015-130, Santa Catarina, Brazil., Ioshii SO; School of Medicine, Pontifícia Universidade Católica do Paraná, Curitiba 80215-901, Paraná, Brazil., de Lima Alves GN; School of Medicine, Pontifícia Universidade Católica do Paraná, Curitiba 80215-901, Paraná, Brazil., Pigatto RN; School of Medicine, Pontifícia Universidade Católica do Paraná, Curitiba 80215-901, Paraná, Brazil., Pinto RL; School of Medicine, Pontifícia Universidade Católica do Paraná, Curitiba 80215-901, Paraná, Brazil., de Carvalho KAT; Pelé Pequeno Príncipe Institute, Child and Adolescent Health Research & Pequeno Príncipe Faculty, Curitiba 80250-060, Paraná, Brazil., Francisco JC; Positivo School of Medicine, Curitiba 81280-330, Paraná, Brazil., Guarita-Souza LC; School of Medicine, Pontifícia Universidade Católica do Paraná, Curitiba 80215-901, Paraná, Brazil. |
| Abstrakt: |
Introduction: Most transplanted organs are obtained from brain-dead donors. Inflammation results in a higher rate of rejection. Objectives: The objective of this animal model of brain death (BD) was to evaluate the effect of the progressive institution of volume expansion, norepinephrine, and combined hormone therapy on clinical, laboratory, and histological aspects. Methods: Twenty rabbits were divided: A (control), B (induction of BD + infusion of crystalloid), C (BD + infusion of crystalloid and noradrenaline (NA)), and D (BD + infusion of crystalloid + vasopressin + levothyroxine + methylprednisolone + NA). The animals were monitored for four hours with consecutives analysis of vital signs and blood samples. The organs were evaluated by a pathologist. Results: In Group D, we observed fewer number and lesser volume of infusions ( p = 0.032/0.014) when compared with groups B and C. Mean arterial pressure levels were higher in group D when compared with group B ( p = 0.008). Group D had better glycemic control when compared with group C ( p = 0.016). Sodium values were elevated in group B in relation to groups C and D ( p = 0.021). In Group D, the organ perfusion was better. Conclusion: The optimized strategy of management of BD animals is associated with better hemodynamic, glycemic, and natremia control, besides reducing early signs of ischemia. |
