| Autor: |
Rocha MP; Departament of Pharmaceutical Products, Faculty of Pharmacy, Universidade Federal de Minas Gerais, Campus Pampulha, Avenida Antônio Carlos 6627, Belo Horizonte 31270-901, MG, Brazil., da Silva LMC; Departament of Pharmaceutical Products, Faculty of Pharmacy, Universidade Federal de Minas Gerais, Campus Pampulha, Avenida Antônio Carlos 6627, Belo Horizonte 31270-901, MG, Brazil., Silva LPM; Departament of Pharmaceutical Products, Faculty of Pharmacy, Universidade Federal de Minas Gerais, Campus Pampulha, Avenida Antônio Carlos 6627, Belo Horizonte 31270-901, MG, Brazil., Gomes JHS; Departament of Pharmaceutical Products, Faculty of Pharmacy, Universidade Federal de Minas Gerais, Campus Pampulha, Avenida Antônio Carlos 6627, Belo Horizonte 31270-901, MG, Brazil., Pádua RM; Departament of Pharmaceutical Products, Faculty of Pharmacy, Universidade Federal de Minas Gerais, Campus Pampulha, Avenida Antônio Carlos 6627, Belo Horizonte 31270-901, MG, Brazil., Batista JAN; Departament of Botany and Molecular Biology, Universidade Federal de Minas Gerais, Campus Pampulha, Avenida Antônio Carlos 6627, Belo Horizonte 31270-901, MG, Brazil., Sena MM; Chemistry Department, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, Belo Horizonte 31270-901, MG, Brazil.; Instituto Nacional de Ciência e Tecnologia em Bioanalítica (INCT-Bio), Campinas 13083-970, SP, Brazil., Campana PRV; Departament of Pharmaceutical Products, Faculty of Pharmacy, Universidade Federal de Minas Gerais, Campus Pampulha, Avenida Antônio Carlos 6627, Belo Horizonte 31270-901, MG, Brazil., Braga FC; Departament of Pharmaceutical Products, Faculty of Pharmacy, Universidade Federal de Minas Gerais, Campus Pampulha, Avenida Antônio Carlos 6627, Belo Horizonte 31270-901, MG, Brazil. |
| Abstrakt: |
This study investigated the similarities between Echinodorus macrophyllus and Echinodorus grandiflorus , plant species that are traditionally used in Brazil to treat rheumatism and arthritis, whose anti-inflammatory effects are supported by scientific evidence. The contents of cis - and trans -aconitic acid, homoorientin, chicoric acid, swertisin, caffeoyl-feruloyl-tartaric acid, and di-feruloyl-tartaric acid were quantified by UPLC-DAD in various hydroethanolic extracts from the leaves, whereas their anti-oxidant activity and their effect on TNF release by LPS-stimulated THP-1 cells were assessed to evaluate potential anti-inflammatory effects. The 50% and 70% ethanol extracts showed higher concentrations of the analyzed markers in two commercial samples and a cultivated specimen of E. macrophyllus , as well as in a commercial lot of E. grandiflorus . However, distinguishing between the species based on marker concentrations was not feasible. The 50% and 70% ethanol extracts also exhibited higher biological activity, yet they did not allow differentiation between the species, indicating similar chemical composition and biological effects. Principal component analysis highlighted comparable chemical composition and biological activity among the commercial samples of E. macrophyllus , while successfully distinguishing the cultivated specimen from the commercial lots. In summary, no differences were observed between the two species in terms of the evaluated chemical markers and biological activities. |
