| Autor: |
Cordeiro-Massironi K; Department of Nutrition, School of Public Health, University of São Paulo, São Paulo 01246-904, Brazil., Soares-Freitas RAM; Department of Nutrition, School of Public Health, University of São Paulo, São Paulo 01246-904, Brazil., Sampaio GR; Department of Nutrition, School of Public Health, University of São Paulo, São Paulo 01246-904, Brazil., Pinaffi-Langley ACDC; Department of Nutrition Sciences, College of Allied Health, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73117, USA., Bridi R; Departamento de Química Farmacológica y Toxicológica, Facultad de Ciencias Químicas y Farmacéuticas, Universidad de Chile, Santiago 8380000, Chile., de Camargo AC; Nutrition and Food Technology Institute, University of Chile, Santiago 7830490, Chile., Torres EAFS; Department of Nutrition, School of Public Health, University of São Paulo, São Paulo 01246-904, Brazil. |
| Abstrakt: |
Peanut skin is a rich source of bioactive compounds which may be able to reduce the risk factors associated with metabolic syndromes. This study aimed to characterize bio-compounds from peanut skin ( Arachis hypogaea ) and their bioactivity (antioxidant activity, inhibition of lipase, and carbohydrase enzymes) and to evaluate their anti-proliferative properties in colorectal cancer cells (HCT116) upon in vitro digestion. Peanut skin was digested in two sequential phases, and the final content, named phase-1 (P1) and phase-2 (P2) extracts, was evaluated. Several bioactive compounds were positively identified and quantified by liquid chromatography, including quinic acid, released especially after in vitro digestion. The total phenolic content and, regardless of the method, the antioxidant activity of P1 was higher than P2. P1 also showed a lower enzyme inhibitory concentration IC 50 than P2, lipase, and α-glucosidase. For cell viability in HCT116 cells, lower concentrations of P1 were found for IC 50 compared to P2. In conclusion, bioactive compounds were released mainly during the first phase of the in vitro digestion. The digested samples presented antioxidant activity, enzyme inhibitory activity, and cancer cell cytotoxicity, especially those from the P1 extract. The potential applications of such a by-product in human health are reported. |
