Etiology behind canine uterine inertia: Role of uterine expression of MLCK4, MYH2, and PKC genes.

Autor: P K M; Department of Animal Reproduction Gynaecology and Obstetrics, College of Veterinary and Animal Sciences Mannuthy, Kerala Veterinary and Animal Sciences University, Thrissur, Kerala, India. Electronic address: magnus@kvasu.ac.in., C J; Department of Animal Reproduction Gynaecology and Obstetrics, College of Veterinary and Animal Sciences Mannuthy, Kerala Veterinary and Animal Sciences University, Thrissur, Kerala, India., T N; Department of Animal Breeding and Genetics, College of Veterinary and Animal Sciences Mannuthy, Kerala Veterinary and Animal Sciences University, Thrissur, Kerala, India., F A L; Department of Animal Breeding and Genetics, College of Veterinary and Animal Sciences Mannuthy, Kerala Veterinary and Animal Sciences University, Thrissur, Kerala, India., M Harshan H; Department of Animal Reproduction Gynaecology and Obstetrics, College of Veterinary and Animal Sciences Mannuthy, Kerala Veterinary and Animal Sciences University, Thrissur, Kerala, India., R S A; Department of Animal Reproduction Gynaecology and Obstetrics, College of Veterinary and Animal Sciences Mannuthy, Kerala Veterinary and Animal Sciences University, Thrissur, Kerala, India., S A; University Veterinary Hospital and Teaching Veterinary Clinical Complex, Mannuthy, Kerala Veterinary and Animal Sciences University, Thrissur, Kerala, India.
Jazyk: angličtina
Zdroj: Animal reproduction science [Anim Reprod Sci] 2023 Sep; Vol. 256, pp. 107298. Date of Electronic Publication: 2023 Jul 20.
DOI: 10.1016/j.anireprosci.2023.107298
Abstrakt: Dystocia is an obstetrical emergency, and primary uterine inertia (PUI) is the major etiological reason among the more prevalent maternal causes in dogs. The present study involved the relative expression analysis of genes associated with myometrial contraction in medium-sized dog breeds with uterine inertia. Dogs without any progress in the parturition process even after four hours of the onset of labor and the absence of uterine contractions were considered to have complete primary uterine inertia (CPUI, n = 9). Dogs that had expelled at least one fetus and made no further progress in parturition in the absence of active uterine contraction were considered to be experiencing partial primary uterine inertia (PPUI, n = 6). Dogs with the fetal cause of dystocia (FCD), i.e., obstructive dystocia, were taken as the third (n = 7) group. Uterine tissue samples were collected during cesarean section in each group, RNA was isolated, and the relative expression of myometrial ACTA2, ACTG2, MLCK4, MYH2, and PKC genes was analyzed. The MLCK4 gene expression was downregulated in CPUI (P ≤ 0.05) and PPUI (P ≤ 0.01) when compared to FCD. The MYH2 gene expression was downregulated in PPUI in comparison to CPUI (P ≤ 0.01) and FCD (P ≤ 0.05). The PKC gene expression was upregulated in PPUI in comparison to FCD and CPUI (P ≤ 0.05). The downregulation of MLCK4 and MYH2 gene expressions recorded in PPUI indicated the possibility of myometrial defects. The possibility of myometrial defects was also observed in CPUI, but to a lesser degree, suggesting other etiologies.
Competing Interests: Declaration of Competing Interest The authors declare that they have no competing interests.
(Copyright © 2023 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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