Natural cordiaquinones as strategies to inhibit the growth and biofilm formation of methicillin-sensitive and methicillin-resistant Staphylococcus spp.

Autor:	de Oliveira MA; Universidade Federal do Delta do Parnaíba, Núcleo de Pesquisa e Pos-graduação, Parnaíba, PI 64202-020, Brazil., Barros AB; Universidade Federal do Delta do Parnaíba, Núcleo de Pesquisa e Pos-graduação, Parnaíba, PI 64202-020, Brazil., de Araújo GS; Universidade Federal do Delta do Parnaíba, Núcleo de Pesquisa e Pos-graduação, Parnaíba, PI 64202-020, Brazil., de Araújo AR; Universidade Federal do Delta do Parnaíba, Núcleo de Pesquisa e Pos-graduação, Parnaíba, PI 64202-020, Brazil., José Dos Santos Soares M; Universidade Federal do Piauí, Departamento de Morfofisiologia Veterinária, Teresina, PI 64049-550, Brazil., de Oliveira DF; Instituto Federal de Educação, Ciência e Tecnologia de São Paulo, Departamento de Física,Matão, SP 15991502, Brazil., Lima FCDA; Instituto Federal de Educação, Ciência e Tecnologia de São Paulo, Departamento de Física,Matão, SP 15991502, Brazil., Batagin-Neto A; Universidade Estadual Paulista, Campus de Itapeva, Departamento de Ciências e Tecnologia, Itapeva, SP 18409-010, Brazil., Leite JRSA; Universidade de Brasília, Departamento de Morfologia, Brasília, DF 70910-900, Brazil., Cesário HPSF; Universidade Federal do Ceará, Departamento de Quimica Orgânica e Inorgânica, Fortaleza, CE 60021-970, Brazil., Pessoa ODL; Universidade Federal do Ceará, Departamento de Quimica Orgânica e Inorgânica, Fortaleza, CE 60021-970, Brazil., Filho JDBM; Universidade Federal do Delta do Parnaíba, Núcleo de Pesquisa e Pos-graduação, Parnaíba, PI 64202-020, Brazil., Araújo AJ; Universidade Federal do Delta do Parnaíba, Núcleo de Pesquisa e Pos-graduação, Parnaíba, PI 64202-020, Brazil.
Jazyk:	angličtina
Zdroj:	Journal of applied microbiology [J Appl Microbiol] 2023 Aug 01; Vol. 134 (8).
DOI:	10.1093/jambio/lxad162
Abstrakt:	Aims: The aim of this study was to investigate the antibacterial and antibiofilm potential of cordiaquinones B, E, L, N, and O against different Staphylococci strains, in addition to analyzing in silico the observed effect. Methods and Results: The minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) were determined according to CLSI guidelines. The inhibition of biofilm formation was investigated at sub-MICs. Atomic force microscopy (AFM) and density functional theory method were performed. The tested strains of Staphylococcus spp. were susceptible to cordiaquinones B, E, and L, among which cordiaquinone B exerted a bactericidal effect, confirmed by a bacterial growth curve study, against Staphylococcus saprophyticus. Cordiaquinones B and E showed lowest MBC values against S. saprophyticus. AFM revealed that cordiaquinone L reduced the mean cell size of S. saprophyticus. Cordiaquinones B and E inhibited the biofilm formation ability of S. aureus by ∼90%. The in silico analysis suggested that the antimicrobial activity of cordiaquinones is driven by their electron donation capability. Conclusions: Cordiaquinones inhibit the growth and biofilm formation (virulence factor) of both methicillin-sensitive and methicillin-resistant Staphylococci strains, indicating their antimicrobial potential. (© The Author(s) 2023. Published by Oxford University Press on behalf of Applied Microbiology International.)
Databáze:	MEDLINE
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