Titanium coated with 2-decenoic analogs reduces bacterial and fungal biofilms.

Autor:	Harrison ZL; Biomedical Engineering, The University of Memphis, Memphis, TN 38152, United States., Montgomery EC; Biomedical Engineering, The University of Memphis, Memphis, TN 38152, United States., Hoffman B; Chemistry, The University of Memphis, Memphis, TN 38152, United States., Perez F; Integrated Microscopy Center, The University of Memphis, Memphis, TN 38152, United States., Bush JR; Biomedical Engineering, The University of Memphis, Memphis, TN 38152, United States., Bumgardner JD; Biomedical Engineering, The University of Memphis, Memphis, TN 38152, United States., Fujiwara T; Chemistry, The University of Memphis, Memphis, TN 38152, United States., Baker DL; Chemistry, The University of Memphis, Memphis, TN 38152, United States., Jennings JA; Biomedical Engineering, The University of Memphis, Memphis, TN 38152, United States.
Jazyk:	angličtina
Zdroj:	Journal of applied microbiology [J Appl Microbiol] 2023 Aug 01; Vol. 134 (8).
DOI:	10.1093/jambio/lxad155
Abstrakt:	Aims: Due to antibiotic tolerance of microbes within biofilm, non-antibiotic methods for prevention and treatment of implant-related infections are preferable. The goal of this work is to evaluate a facile loading strategy for medium-chain fatty-acid signaling molecules 2-heptycyclopropane-1-carboxylic acid (2CP), cis-2-decenoic acid (C2DA), and trans-2-decenoic acid, which all act as diffusible signaling factors (DSFs), onto titanium surfaces for comparison of their antimicrobial efficacy. Methods and Results: Titanium coupons were drop-coated with 0.75 mg of DSF in ethanol and dried. Surface characteristics and the presence of DSF were confirmed with Fourier Transform infrared spectroscopy, x-ray photoelectron spectroscopy, and water contact angle. Antimicrobial assays analyzing biofilm and planktonic Staphylococcus aureus, Escherichia coli, or Candida albicans viability showed that planktonic growth was reduced after 24-h incubation but only sustained through 72 h for S. aureus and C. albicans. Biofilm formation on the titanium coupons was also reduced for all strains at the 24-h time point, but not through 72 h for E. coli. Although ∼60% of the loaded DSF was released within the first 2 days, enough remained on the surface after 4 days of elution to significantly inhibit E. coli and C. albicans biofilm. Cytocompatibility evaluations with a fibroblast cell line showed that none of the DSF-loaded groups decreased viability, while C2DA and 2CP increased viability by up to 50%. Conclusions: In this study, we found that DSF-loaded titanium coupons can inhibit planktonic microbes and prevent biofilm attachment, without toxicity to mammalian cells. (© The Author(s) 2023. Published by Oxford University Press on behalf of Applied Microbiology International.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu