Efficient production of bacterial antibiotics aminoriboflavin and roseoflavin in eukaryotic microorganisms, yeasts.

Autor: Dmytruk KV; Institute of Cell Biology, National Academy of Sciences of Ukraine, Drahomanov St, 14/16, Lviv, 79005, Ukraine., Ruchala J; University of Rzeszow, Zelwerowicza 4, Rzeszow, 35-601, Poland., Fayura LR; Institute of Cell Biology, National Academy of Sciences of Ukraine, Drahomanov St, 14/16, Lviv, 79005, Ukraine., Chrzanowski G; University of Rzeszow, Zelwerowicza 4, Rzeszow, 35-601, Poland., Dmytruk OV; Institute of Cell Biology, National Academy of Sciences of Ukraine, Drahomanov St, 14/16, Lviv, 79005, Ukraine., Tsyrulnyk AO; Institute of Cell Biology, National Academy of Sciences of Ukraine, Drahomanov St, 14/16, Lviv, 79005, Ukraine., Andreieva YA; Institute of Cell Biology, National Academy of Sciences of Ukraine, Drahomanov St, 14/16, Lviv, 79005, Ukraine., Fedorovych DV; Institute of Cell Biology, National Academy of Sciences of Ukraine, Drahomanov St, 14/16, Lviv, 79005, Ukraine., Motyka OI; Research Institute of Epidemiology and Hygiene of the Danylo Halytsky Lviv National Medical University, Zelena St, 12, Lviv, 79005, Ukraine., Mattanovich D; Department of Biotechnology, Institute of Microbiology and Microbial Biotechnology, University of Natural Resources and Life Sciences, Muthgasse 18, Vienna, Vienna, 1190, Austria., Marx H; Department of Biotechnology, Institute of Microbiology and Microbial Biotechnology, University of Natural Resources and Life Sciences, Muthgasse 18, Vienna, Vienna, 1190, Austria., Sibirny AA; Institute of Cell Biology, National Academy of Sciences of Ukraine, Drahomanov St, 14/16, Lviv, 79005, Ukraine. sibirny@yahoo.com.; University of Rzeszow, Zelwerowicza 4, Rzeszow, 35-601, Poland. sibirny@yahoo.com.
Jazyk: angličtina
Zdroj: Microbial cell factories [Microb Cell Fact] 2023 Jul 20; Vol. 22 (1), pp. 132. Date of Electronic Publication: 2023 Jul 20.
DOI: 10.1186/s12934-023-02129-8
Abstrakt: Background: Actinomycetes Streptomyces davaonensis and Streptomyces cinnabarinus synthesize a promising broad-spectrum antibiotic roseoflavin, with its synthesis starting from flavin mononucleotide and proceeding through an immediate precursor, aminoriboflavin, that also has antibiotic properties. Roseoflavin accumulation by the natural producers is rather low, whereas aminoriboflavin accumulation is negligible. Yeasts have many advantages as biotechnological producers relative to bacteria, however, no recombinant producers of bacterial antibiotics in yeasts are known.
Results: Roseoflavin biosynthesis genes have been expressed in riboflavin- or FMN-overproducing yeast strains of Candida famata and Komagataella phaffii. Both these strains accumulated aminoriboflavin, whereas only the latter produced roseoflavin. Aminoriboflavin isolated from the culture liquid of C. famata strain inhibited the growth of Staphylococcus aureus (including MRSA) and Listeria monocytogenes. Maximal accumulation of aminoriboflavin in shake-flasks reached 1.5 mg L - 1 (C. famata), and that of roseoflavin was 5 mg L - 1 (K. phaffii). Accumulation of aminoriboflavin and roseoflavin by K. phaffii recombinant strain in a bioreactor reached 22 and 130 mg L - 1 , respectively. For comparison, recombinant strains of the native bacterial producer S. davaonensis accumulated near one-order less of roseoflavin while no recombinant producers of aminoriboflavin was reported at all.
Conclusions: Yeast recombinant producers of bacterial antibiotics aminoriboflavin and roseoflavin were constructed and evaluated.
(© 2023. The Author(s).)
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje