Enabling X-ray fluorescence imaging for in vivo immune cell tracking.

Autor: Staufer T; Fachbereich Physik, Universität Hamburg, 22761, Hamburg, Germany. theresa.staufer@uni-hamburg.de.; Center for Free-Electron Laser Science (CFEL), 22761, Hamburg, Germany. theresa.staufer@uni-hamburg.de., Körnig C; Fachbereich Physik, Universität Hamburg, 22761, Hamburg, Germany.; Center for Free-Electron Laser Science (CFEL), 22761, Hamburg, Germany., Liu B; I. Department of Medicine, University Medical Center Hamburg-Eppendorf, 20246, Hamburg, Germany.; Hamburg Center for Translational Immunology (HCTI), University Medical Center Hamburg-Eppendorf, 20246, Hamburg, Germany., Liu Y; Fachbereich Physik, Universität Hamburg, 22761, Hamburg, Germany.; Center for Hybrid Nanostructures (CHyN), Luruper Chaussee 149, 22761, Hamburg, Germany., Lanzloth C; I. Department of Medicine, University Medical Center Hamburg-Eppendorf, 20246, Hamburg, Germany.; Protozoa Immunology, Bernhard Nocht Institute for Tropical Medicine, 20359, Hamburg, Germany., Schmutzler O; Fachbereich Physik, Universität Hamburg, 22761, Hamburg, Germany.; Center for Free-Electron Laser Science (CFEL), 22761, Hamburg, Germany., Bedke T; I. Department of Medicine, University Medical Center Hamburg-Eppendorf, 20246, Hamburg, Germany.; Hamburg Center for Translational Immunology (HCTI), University Medical Center Hamburg-Eppendorf, 20246, Hamburg, Germany., Machicote A; I. Department of Medicine, University Medical Center Hamburg-Eppendorf, 20246, Hamburg, Germany.; Hamburg Center for Translational Immunology (HCTI), University Medical Center Hamburg-Eppendorf, 20246, Hamburg, Germany., Parak WJ; Fachbereich Physik, Universität Hamburg, 22761, Hamburg, Germany.; Center for Hybrid Nanostructures (CHyN), Luruper Chaussee 149, 22761, Hamburg, Germany., Feliu N; Fraunhofer Center for Applied Nanotechnology (IAP-CAN), 20146, Hamburg, Germany., Bosurgi L; I. Department of Medicine, University Medical Center Hamburg-Eppendorf, 20246, Hamburg, Germany.; Hamburg Center for Translational Immunology (HCTI), University Medical Center Hamburg-Eppendorf, 20246, Hamburg, Germany.; Protozoa Immunology, Bernhard Nocht Institute for Tropical Medicine, 20359, Hamburg, Germany., Huber S; I. Department of Medicine, University Medical Center Hamburg-Eppendorf, 20246, Hamburg, Germany.; Hamburg Center for Translational Immunology (HCTI), University Medical Center Hamburg-Eppendorf, 20246, Hamburg, Germany., Grüner F; Fachbereich Physik, Universität Hamburg, 22761, Hamburg, Germany.; Center for Free-Electron Laser Science (CFEL), 22761, Hamburg, Germany.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2023 Jul 17; Vol. 13 (1), pp. 11505. Date of Electronic Publication: 2023 Jul 17.
DOI: 10.1038/s41598-023-38536-5
Abstrakt: The infiltration of immune cells into sites of inflammation is one key feature of immune mediated inflammatory diseases. A detailed assessment of the in vivo dynamics of relevant cell subtypes could booster the understanding of this disease and the development of novel therapies. We show in detail how advanced X-ray fluorescence imaging enables such quantitative in vivo cell tracking, offering solutions that could pave the way beyond what other imaging modalities provide today. The key for this achievement is a detailed study of the spectral background contribution from multiple Compton scattering in a mouse-scaled object when this is scanned with a monochromatic pencil X-ray beam from a synchrotron. Under optimal conditions, the detection sensitivity is sufficient for detecting local accumulations of the labelled immune cells, hence providing experimental demonstration of in vivo immune cell tracking in mice.
(© 2023. The Author(s).)
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje