Raman, WAXS, and Solid-State NMR Characterizations of Regenerated Silk Fibroin Using Lanthanide Ions as Chaotropic Agents.

Autor:	Rizzo G; Dipartimento di Chimica, Università degli Studi di Bari Aldo Moro, via Orabona 4, 70125 Bari, Italy., Petrelli V; DICATECh, Politecnico di Bari, via Orabona 4, 70125 Bari, Italy., Sibillano T; CNR IC-Institute of Crystallography, via Amendola 122/O, Bari 70126, Italy., De Caro L; CNR IC-Institute of Crystallography, via Amendola 122/O, Bari 70126, Italy., Giangregorio MM; Institute of Nanotechnology, CNR NANOTEC, c/o, Dipartimento di Chimica, Università di Bari, via Orabona 4, 70126 Bari, Italy., Lo Presti M; Silklab, Department of Biomedical Engineering, Tufts University, 200 Boston Avenue, Suite 4875, Medford, Massachusetts 02155, United States., Omenetto FG; Silklab, Department of Biomedical Engineering, Tufts University, 200 Boston Avenue, Suite 4875, Medford, Massachusetts 02155, United States., Giannini C; CNR IC-Institute of Crystallography, via Amendola 122/O, Bari 70126, Italy., Mastrorilli P; DICATECh, Politecnico di Bari, via Orabona 4, 70125 Bari, Italy., Farinola GM; Dipartimento di Chimica, Università degli Studi di Bari Aldo Moro, via Orabona 4, 70125 Bari, Italy.; Silklab, Department of Biomedical Engineering, Tufts University, 200 Boston Avenue, Suite 4875, Medford, Massachusetts 02155, United States.
Jazyk:	angličtina
Zdroj:	ACS omega [ACS Omega] 2023 Jun 28; Vol. 8 (27), pp. 24165-24175. Date of Electronic Publication: 2023 Jun 28 (Print Publication: 2023).
DOI:	10.1021/acsomega.2c07149
Abstrakt:	Bombyx mori silk fibroin (SF) has been reported as a convenient natural material for regenerative medicine, optoelectronics, and many other technological applications. SF owes its unique features to the hierarchical organization of the fibers. Many efforts have been made to set up protocols for dissolution since many applications of SF are based on regenerated solutions and fibers, but chaotropic conditions required to disassemble the packing of the polymer afford solutions with poor crystalline behavior. Our previous research has disclosed a dissolution and regeneration process of highly crystalline fibers involving lanthanide ions as chaotropic agents, demonstrating that each lanthanide has its own unique interaction with SF. Herein, we report elucidation of the structure of Ln-SF fibers by the combined use of Raman spectroscopy, wide-angle X-ray scattering (WAXS), and solid-state NMR techniques. Raman spectra confirmed the coordination of metal ions to SF, WAXS results highlighted the crystalline content of fibers, and solid-state NMR enabled the assessment of different ratios of secondary structures in the protein. Competing Interests: The authors declare no competing financial interest. (© 2023 The Authors. Published by American Chemical Society.)
Databáze:	MEDLINE
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